PUBLICATION
Whole-Organism Screening for Modulators of Fasting Metabolism Using Transgenic Zebrafish
- Authors
- Gut, P., Stainier, D.Y.
- ID
- ZDB-PUB-150127-8
- Date
- 2015
- Source
- Methods in molecular biology (Clifton, N.J.) 1263: 157-165 (Other)
- Registered Authors
- Gut, Philipp, Stainier, Didier
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Drug Evaluation, Preclinical/methods*
- Energy Metabolism/drug effects*
- Fasting/metabolism*
- Luminescent Measurements/methods
- Small Molecule Libraries
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 25618343 Full text @ Meth. Mol. Biol.
Citation
Gut, P., Stainier, D.Y. (2015) Whole-Organism Screening for Modulators of Fasting Metabolism Using Transgenic Zebrafish. Methods in molecular biology (Clifton, N.J.). 1263:157-165.
Abstract
Organismal energy homeostasis is maintained by complex interorgan communications making the discovery of novel drugs against metabolic diseases challenging using traditional high-throughput approaches in vitro. Here, we describe a method that rapidly identifies small molecules with an impact on organismal energy balance in vivo. Specifically, we developed a whole-organism screen for modulators of fasting metabolism using transgenic bioluminescence-reporter zebrafish for the gluconeogenic gene phosphoenolpyruvate-carboxykinase 1 (pck1).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping