PUBLICATION
Making sense of anti-sense data
- Authors
- Stainier, D.Y., Kontarakis, Z., Rossi, A.
- ID
- ZDB-PUB-150115-10
- Date
- 2015
- Source
- Developmental Cell 32: 7-8 (Other)
- Registered Authors
- Kontarakis, Zacharias, Stainier, Didier
- Keywords
- none
- MeSH Terms
-
- Animals
- Gene Expression Regulation/drug effects*
- Gene Knockdown Techniques/methods*
- Morpholinos/pharmacology*
- Oligonucleotides, Antisense/pharmacology*
- Phenotype
- Zebrafish/genetics*
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/genetics*
- PubMed
- 25584794 Full text @ Dev. Cell
Citation
Stainier, D.Y., Kontarakis, Z., Rossi, A. (2015) Making sense of anti-sense data. Developmental Cell. 32:7-8.
Abstract
The morpholino anti-sense technology has been used extensively to test gene function. The zebrafish model allows a detailed comparison of knockdown (anti-sense) and knockout (mutation) effects. Recent studies reveal that these two approaches can often lead to surprisingly different phenotypes, thus raising a number of important questions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping