Stainier, D.Y., Kontarakis, Z., Rossi, A. (2015) Making sense of anti-sense data. Developmental Cell. 32:7-8.
The morpholino anti-sense technology has been used extensively to test gene function. The zebrafish model allows a detailed comparison of knockdown (anti-sense) and knockout (mutation) effects. Recent studies reveal that these two approaches can often lead to surprisingly different phenotypes, thus raising a number of important questions.