ZFIN ID: ZDB-PUB-150115-10
Making sense of anti-sense data
Stainier, D.Y., Kontarakis, Z., Rossi, A.
Date: 2015
Source: Developmental Cell 32: 7-8 (Other)
Registered Authors: Kontarakis, Zacharias, Stainier, Didier
Keywords: none
MeSH Terms:
  • Animals
  • Gene Expression Regulation/drug effects*
  • Gene Knockdown Techniques/methods*
  • Morpholinos/pharmacology*
  • Oligonucleotides, Antisense/pharmacology*
  • Phenotype
  • Zebrafish/genetics*
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/genetics*
PubMed: 25584794 Full text @ Dev. Cell
The morpholino anti-sense technology has been used extensively to test gene function. The zebrafish model allows a detailed comparison of knockdown (anti-sense) and knockout (mutation) effects. Recent studies reveal that these two approaches can often lead to surprisingly different phenotypes, thus raising a number of important questions.