PUBLICATION

Crypt neurons express a single V1R-related ora gene

Authors
Oka, Y., Saraiva, L.R., Korsching, S.I.
ID
ZDB-PUB-141222-2
Date
2012
Source
Chemical senses   37(3): 219-227 (Journal)
Registered Authors
Korsching, Sigrun, Saraiva, Luis
Keywords
ciliated, crypt cell, inhibitory G protein, microvillous, olfaction, zebrafish
MeSH Terms
  • Animals
  • Gene Expression Regulation
  • In Situ Hybridization
  • Olfactory Receptor Neurons/metabolism*
  • Receptors, Odorant/genetics
  • Receptors, Odorant/metabolism*
  • Signal Transduction
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
22038944 Full text @ Chem. Senses
Abstract
Both ciliated and microvillous olfactory sensory neuron populations express large families of olfactory receptor genes. However, individual neurons generally express only a single receptor gene according to the “one neuron–one receptor” rule. We report here that crypt neurons, the third type of olfactory neurons in fish species, use an even more restricted mode of expression. We recently identified a novel olfactory receptor family of 6 highly conserved G protein–coupled receptors, the v1r-like ora genes. We show now that a single member of this family, ora4 is expressed in nearly all crypt neurons, whereas the other 5 ora genes are not found in this cell type. Consistent with these findings, ora4 is never coexpressed with any of the remaining 5 ora genes. Furthermore, several lines of evidence indicate the absence of any other olfactory receptor families in crypt neurons. These results suggest that the vast majority of the crypt neuron population may select one and the same olfactory receptor gene, a “one cell type–one receptor” mode of expression. Such an expression pattern is familiar in the visual system, with rhodopsin as the sole light receptor of rod photoreceptor cells, but unexpected in the sense of smell.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping