PUBLICATION
The kiss/kissr systems are dispensable for zebrafish reproduction: evidence from gene knockout studies
- Authors
- Tang, H., Liu, Y., Luo, D., Ogawa, S., Yin, Y., Li, S., Zhang, Y., Hu, W., Parhar, I.S., Lin, H., Liu, X., Cheng, C.H.
- ID
- ZDB-PUB-141119-1
- Date
- 2015
- Source
- Endocrinology 156(2): 589-99 (Journal)
- Registered Authors
- Hu, Wei, Liu, Xiaochun, Ogawa, Satoshi, Zhang, Yong
- Keywords
- none
- MeSH Terms
-
- Animals
- Female
- Follicle Stimulating Hormone, beta Subunit/metabolism
- Gene Knockout Techniques
- Gonadotropin-Releasing Hormone/metabolism
- Kisspeptins/genetics
- Kisspeptins/metabolism*
- Luteinizing Hormone, beta Subunit/metabolism
- Male
- Receptors, G-Protein-Coupled/genetics
- Receptors, G-Protein-Coupled/metabolism*
- Reproduction*
- Sexual Maturation
- Zebrafish/physiology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 25406015 Full text @ Endocrinology
Citation
Tang, H., Liu, Y., Luo, D., Ogawa, S., Yin, Y., Li, S., Zhang, Y., Hu, W., Parhar, I.S., Lin, H., Liu, X., Cheng, C.H. (2015) The kiss/kissr systems are dispensable for zebrafish reproduction: evidence from gene knockout studies. Endocrinology. 156(2):589-99.
Abstract
The kiss1/gpr54 signaling system is considered to be a critical regulator of reproduction in most vertebrates. However this presumption has not been tested vigorously in non-mammalian vertebrates. Distinct from mammals, multiple kiss1/gpr54 paralogous genes (kiss/kissr) have been identified in non-mammalian vertebrates, raising the possibility of functional redundancy among these genes. In this study, we have systematically generated the zebrafish kiss1(-/-), kiss2(-/-) and kiss1(-/-);kiss2(-/-) mutant lines as well as the kissr1(-/-), kissr2(-/-) and kissr1(-/-);kissr2(-/-) mutant lines using transcription activator like effector nucleases (TALENs). We have demonstrated that spermatogenesis and folliculogenesis as well as reproductive capability are not impaired in all of these six mutant lines. Collectively, our results indicate that kiss/kissr signaling is not absolutely required for zebrafish reproduction, suggesting that the kiss/kissr systems play non-essential roles for reproduction in certain non-mammalian vertebrates. These findings also demonstrated that fish and mammals have evolved different strategies for neuroendocrine control of reproduction.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping