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ZFIN ID: ZDB-PUB-141114-8
Barley beta-glucan promotes MnSOD expression and enhances angiogenesis under oxidative microenvironment
Agostini, S., Chiavacci, E., Matteucci, M., Torelli, M., Pitto, L., Lionetti, V.
Date: 2015
Source: Journal of Cellular and Molecular Medicine   19(1): 227-38 (Journal)
Registered Authors: Chiavacci, Elena
Keywords: angiogenesis, antioxidants, beta-glucan, endothelial cells, histone deacetylases
MeSH Terms:
  • Acetylation/drug effects
  • Animals
  • Butyric Acid/pharmacology
  • Capillaries/drug effects
  • Capillaries/physiology
  • Cell Survival/drug effects
  • Cellular Microenvironment/drug effects*
  • Down-Regulation/drug effects
  • Histones/metabolism
  • Hordeum/chemistry*
  • Human Umbilical Vein Endothelial Cells/cytology*
  • Human Umbilical Vein Endothelial Cells/drug effects
  • Human Umbilical Vein Endothelial Cells/enzymology*
  • Human Umbilical Vein Endothelial Cells/metabolism
  • Humans
  • Neovascularization, Physiologic/drug effects*
  • Nitric Oxide/metabolism
  • Nitric Oxide Synthase Type III/metabolism
  • Oxidation-Reduction/drug effects
  • Oxidative Stress/drug effects
  • Phosphorylation/drug effects
  • Superoxide Dismutase/metabolism*
  • Up-Regulation/drug effects
  • Zebrafish
  • beta-Glucans/pharmacology*
PubMed: 25388628 Full text @ J. Cell. Mol. Med.
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ABSTRACT
Manganese superoxide dismutase (MnSOD), a foremost antioxidant enzyme, plays a key role in angiogenesis. Barley-derived (1.3) β-d-glucan (β-d-glucan) is a natural water-soluble polysaccharide with antioxidant properties. To explore the effects of β-d-glucan on MnSOD-related angiogenesis under oxidative stress, we tested epigenetic mechanisms underlying modulation of MnSOD level in human umbilical vein endothelial cells (HUVECs) and angiogenesis in vitro and in vivo. Long-term treatment of HUVECs with 3% w/v β-d-glucan significantly increased the level of MnSOD by 200% ± 2% compared to control and by 50% ± 4% compared to untreated H2 O2 -stressed cells. β-d-glucan-treated HUVECs displayed greater angiogenic ability. In vivo, 24 hrs-treatment with 3% w/v β-d-glucan rescued vasculogenesis in Tg (kdrl: EGFP) s843Tg zebrafish embryos exposed to oxidative microenvironment. HUVECs overexpressing MnSOD demonstrated an increased activity of endothelial nitric oxide synthase (eNOS), reduced load of superoxide anion (O2 (-) ) and an increased survival under oxidative stress. In addition, β-d-glucan prevented the rise of hypoxia inducible factor (HIF)1-α under oxidative stress. The level of histone H4 acetylation was significantly increased by β-d-glucan. Increasing histone acetylation by sodium butyrate, an inhibitor of class I histone deacetylases (HDACs I), did not activate MnSOD-related angiogenesis and did not impair β-d-glucan effects. In conclusion, 3% w/v β-d-glucan activates endothelial expression of MnSOD independent of histone acetylation level, thereby leading to adequate removal of O2 (-) , cell survival and angiogenic response to oxidative stress. The identification of dietary β-d-glucan as activator of MnSOD-related angiogenesis might lead to the development of nutritional approaches for the prevention of ischemic remodelling and heart failure.
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