PUBLICATION

Translational profiling through biotinylation of tagged ribosomes in zebrafish

Authors
Housley, M.P., Reischauer, S., Dieu, M., Raes, M., Stainier, D.Y., Vanhollebeke, B.
ID
ZDB-PUB-140919-5
Date
2014
Source
Development (Cambridge, England)   141(20): 3988-93 (Journal)
Registered Authors
Housley, Michael, Reischauer, Sven, Stainier, Didier, Vanhollebeke, Benoit
Keywords
none
Datasets
GEO:GSE59355
MeSH Terms
  • Coenzyme A Ligases/chemistry
  • Transcription, Genetic*
  • Gene Expression Profiling*
  • Animals
  • Gene Expression Regulation, Developmental*
  • Zebrafish Proteins/physiology*
  • Mass Spectrometry
  • Muscle, Skeletal/pathology
  • Zebrafish
  • RNA-Binding Proteins/physiology*
  • In Situ Hybridization
  • Polyribosomes/chemistry
  • Ribosomal Proteins/physiology
  • Biotinylation
  • Green Fluorescent Proteins/chemistry
  • Animals, Genetically Modified
  • Ribosomes/metabolism
  • RNA, Messenger/metabolism
(all 18)
PubMed
25231762 Full text @ Development
Abstract
Heterogeneity within a population of cells of the same type is a common theme in metazoan biology. Dissecting complex developmental and physiological processes crucially relies on our ability to probe the expression profile of these cell subpopulations. Current strategies rely on cell enrichment based on sequential or simultaneous use of multiple intersecting markers starting from a heterogeneous cell suspension. The extensive tissue manipulations required to generate single-cell suspensions, as well as the complexity of the required equipment, inherently complicate these approaches. Here, we propose an alternative methodology based on a genetically encoded system in the model organism Danio rerio (zebrafish). In transgenic fish, we take advantage of the combinatorial biotin transfer system, where polysome-associated mRNAs are selectively recovered from cells expressing both a tagged ribosomal subunit, Rpl10a, and the bacterial biotin ligase BirA. We have applied this technique to skeletal muscle development and identified new genes with interesting temporal expression patterns. Through this work we have thus developed additional tools for highly specific gene expression profiling.
Genes / Markers
Figures
Figure Gallery (3 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
s972TgTransgenic Insertion
    s973TgTransgenic Insertion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      Fish
      s972Tg
      s973Tg
      WT
      1 - 3 of 3
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      Antibodies
      No data available
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      EGFPEFGEGFP
      1 - 1 of 1
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      Mapping
      No data available
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      Citation
      Housley, M.P., Reischauer, S., Dieu, M., Raes, M., Stainier, D.Y., Vanhollebeke, B. (2014) Translational profiling through biotinylation of tagged ribosomes in zebrafish. Development (Cambridge, England). 141(20):3988-93.