PUBLICATION
Stab wound injury of the zebrafish adult telencephalon: a method to investigate vertebrate brain neurogenesis and regeneration
- Authors
- Schmidt, R., Beil, T., Strähle, U., Rastegar, S.
- ID
- ZDB-PUB-140826-16
- Date
- 2014
- Source
- Journal of visualized experiments : JoVE (90): e51753 (Journal)
- Registered Authors
- Beil, Tanja, Rastegar, Sepand, Rodriguez Viales, Rebecca, Strähle, Uwe
- Keywords
- none
- MeSH Terms
-
- Animals
- Immunohistochemistry
- In Situ Hybridization
- Nerve Regeneration/physiology*
- Neurogenesis/physiology*
- Telencephalon/injuries*
- Telencephalon/physiopathology*
- Wound Healing/physiology
- Zebrafish
- PubMed
- 25146302 Full text @ J. Vis. Exp.
Citation
Schmidt, R., Beil, T., Strähle, U., Rastegar, S. (2014) Stab wound injury of the zebrafish adult telencephalon: a method to investigate vertebrate brain neurogenesis and regeneration. Journal of visualized experiments : JoVE. (90):e51753.
Abstract
Adult zebrafish have an amazing capacity to regenerate their central nervous system after injury. To investigate the cellular response and the molecular mechanisms involved in zebrafish adult central nervous system (CNS) regeneration and repair, we developed a zebrafish model of adult telencephalic injury. In this approach, we manually generate an injury by pushing an insulin syringe needle into the zebrafish adult telencephalon. At different post injury days, fish are sacrificed, their brains are dissected out and stained by immunohistochemistry and/or in situ hybridization (ISH) with appropriate markers to observe cell proliferation, gliogenesis, and neurogenesis. The contralateral unlesioned hemisphere serves as an internal control. This method combined for example with RNA deep sequencing can help to screen for new genes with a role in zebrafish adult telencephalon neurogenesis, regeneration, and repair.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping