ZFIN ID: ZDB-PUB-140826-16
Stab wound injury of the zebrafish adult telencephalon: a method to investigate vertebrate brain neurogenesis and regeneration
Schmidt, R., Beil, T., Strähle, U., Rastegar, S.
Date: 2014
Source: Journal of visualized experiments : JoVE   (90): e51753 (Journal)
Registered Authors: Beil, Tanja, Rastegar, Sepand, Rodriguez Viales, Rebecca, Strähle, Uwe
Keywords: none
MeSH Terms:
  • Animals
  • Immunohistochemistry
  • In Situ Hybridization
  • Nerve Regeneration/physiology*
  • Neurogenesis/physiology*
  • Telencephalon/injuries*
  • Telencephalon/physiopathology*
  • Wound Healing/physiology
  • Zebrafish
PubMed: 25146302 Full text @ J. Vis. Exp.
ABSTRACT
Adult zebrafish have an amazing capacity to regenerate their central nervous system after injury. To investigate the cellular response and the molecular mechanisms involved in zebrafish adult central nervous system (CNS) regeneration and repair, we developed a zebrafish model of adult telencephalic injury. In this approach, we manually generate an injury by pushing an insulin syringe needle into the zebrafish adult telencephalon. At different post injury days, fish are sacrificed, their brains are dissected out and stained by immunohistochemistry and/or in situ hybridization (ISH) with appropriate markers to observe cell proliferation, gliogenesis, and neurogenesis. The contralateral unlesioned hemisphere serves as an internal control. This method combined for example with RNA deep sequencing can help to screen for new genes with a role in zebrafish adult telencephalon neurogenesis, regeneration, and repair.
ADDITIONAL INFORMATION