PUBLICATION
A novel HoxB cluster protein expressed in the hindbrain and pharyngeal arches
- Authors
- Saxena, A., Bronner, M.E.
- ID
- ZDB-PUB-140820-3
- Date
- 2014
- Source
- Genesis (New York, N.Y. : 2000) 52(10): 858-63 (Journal)
- Registered Authors
- Bronner-Fraser, Marianne, Saxena, Ankur
- Keywords
- HoxB cluster, Hoxb4a, fluorescent fusion protein, zebrafish
- MeSH Terms
-
- Bacterial Proteins/metabolism
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism*
- Branchial Region/embryology
- Branchial Region/metabolism*
- Animals, Genetically Modified/embryology
- Animals, Genetically Modified/genetics
- Animals, Genetically Modified/metabolism
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism
- Rhombencephalon/embryology
- Rhombencephalon/metabolism*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Embryo, Nonmammalian/metabolism
- Cytoplasm/genetics
- Cytoplasm/metabolism
- Luminescent Proteins/metabolism
- Animals
- Cell Nucleus/genetics
- Cell Nucleus/metabolism
- Gene Expression Regulation, Developmental
- PubMed
- 25137177 Full text @ Genesis
Citation
Saxena, A., Bronner, M.E. (2014) A novel HoxB cluster protein expressed in the hindbrain and pharyngeal arches. Genesis (New York, N.Y. : 2000). 52(10):858-63.
Abstract
We describe a novel zebrafish line that fluorescently tags a previously unknown protein, CT74a, allowing us to follow its endogenous expression in real time and at subcellular resolution in live embryos. Our results showed that CT74a-Citrine fusion protein is expressed in the developing pharyngeal arches, hindbrain, and fin buds in a pattern highly reminiscent of transcription factors belonging to anterior Hox gene families, including expression in a subset of neuronal nuclei. Consistent with this, splinkerette-PCR revealed that CT74a-Citrine's genomic integration is within the HoxB region, and 3' RACE demonstrated that its downstream coding sequence has no recognizable homology. Thus, CT74a is a previously unknown protein located within the HoxB cluster adjacent to Hoxb4a and is expressed in a Hoxb4a-like pattern.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping