PUBLICATION
Understanding cardiac sarcomere assembly with zebrafish genetics
- Authors
- Yang, J., Shih, Y.H., Xu, X.
- ID
- ZDB-PUB-140816-8
- Date
- 2014
- Source
- Anatomical record (Hoboken, N.J. : 2007) 297: 1681-93 (Review)
- Registered Authors
- Shih, Yu-huan, Xu, Xiaolei, Yang, Jingchun
- Keywords
- cardiomyopathy, genetics, myofibrillogenesis, sarcomere, zebrafish
- MeSH Terms
-
- Animals, Genetically Modified
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Phenotype
- Genotype
- Genetic Predisposition to Disease
- Myocytes, Cardiac/metabolism*
- Myocytes, Cardiac/pathology
- Humans
- Mutation
- Heart Diseases/genetics
- Heart Diseases/metabolism
- Heart Diseases/pathology
- Risk Factors
- Zebrafish/genetics*
- Zebrafish/metabolism
- Animals
- Sarcomeres/metabolism*
- Sarcomeres/pathology
- PubMed
- 25125181 Full text @ Anat. Rec. (Hoboken)
Citation
Yang, J., Shih, Y.H., Xu, X. (2014) Understanding cardiac sarcomere assembly with zebrafish genetics. Anatomical record (Hoboken, N.J. : 2007). 297:1681-93.
Abstract
Mutations in sarcomere genes have been found in many inheritable human diseases, including hypertrophic cardiomyopathy. Elucidating the molecular mechanisms of sarcomere assembly shall facilitate understanding of the pathogenesis of sarcomere-based cardiac disease. Recently, biochemical and genomic studies have identified many new genes encoding proteins that localize to the sarcomere. However, their precise functions in sarcomere assembly and sarcomere-based cardiac disease are unknown. Here, we review zebrafish as an emerging vertebrate model for these studies. We summarize the techniques offered by this animal model to manipulate genes of interest, annotate gene expression, and describe the resulting phenotypes. We survey the sarcomere genes that have been investigated in zebrafish and discuss the potential of applying this in vivo model for larger-scale genetic studies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping