PUBLICATION
3D imaging of transition metals in the zebrafish embryo by X-ray fluorescence microtomography
- Authors
- Bourassa, D., Gleber, S.C., Vogt, S., Yi, H., Will, F., Richter, H., Shin, C.H., Fahrni, C.J.
- ID
- ZDB-PUB-140706-8
- Date
- 2014
- Source
- Metallomics : integrated biometal science 6(9): 1648-55 (Journal)
- Registered Authors
- Shin, Chong
- Keywords
- none
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/metabolism*
- Fluorescence
- Imaging, Three-Dimensional/methods*
- Lasers
- Time Factors
- Transition Elements/metabolism*
- X-Ray Microtomography/methods*
- Zebrafish/embryology*
- PubMed
- 24992831 Full text @ Metallomics
Citation
Bourassa, D., Gleber, S.C., Vogt, S., Yi, H., Will, F., Richter, H., Shin, C.H., Fahrni, C.J. (2014) 3D imaging of transition metals in the zebrafish embryo by X-ray fluorescence microtomography. Metallomics : integrated biometal science. 6(9):1648-55.
Abstract
Synchrotron X-ray fluorescence (SXRF) microtomography has emerged as a powerful technique for the 3D visualization of the elemental distribution in biological samples. The mechanical stability, both of the instrument and the specimen, is paramount when acquiring tomographic projection series. By combining the progressive lowering of temperature method (PLT) with femtosecond laser sectioning, we were able to embed, excise, and preserve a zebrafish embryo at 24 hours post fertilization in an X-ray compatible, transparent resin for tomographic elemental imaging. Based on a data set comprised of 60 projections, acquired with a step size of 2 μm during 100 hours of beam time, we reconstructed the 3D distribution of zinc, iron, and copper using the iterative maximum likelihood expectation maximization (MLEM) reconstruction algorithm. The volumetric elemental maps, which entail over 124 million individual voxels for each transition metal, revealed distinct elemental distributions that could be correlated with characteristic anatomical features at this stage of embryonic development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping