PUBLICATION
Development of a doubly weighted Gerchberg-Saxton algorithm for use in multibeam imaging applications
- Authors
- Poland, S.P., Krstajic, N., Knight, R.D., Henderson, R.K., Ameer-Beg, S.M.
- ID
- ZDB-PUB-140701-5
- Date
- 2014
- Source
- Optics letters 39: 2431-2434 (Journal)
- Registered Authors
- Knight, Robert
- Keywords
- none
- MeSH Terms
-
- Algorithms
- Animals
- Image Processing, Computer-Assisted/statistics & numerical data
- Larva/cytology
- Microscopy, Fluorescence, Multiphoton/instrumentation
- Microscopy, Fluorescence, Multiphoton/methods*
- Microscopy, Fluorescence, Multiphoton/statistics & numerical data
- Muscle Fibers, Skeletal/cytology
- Optical Phenomena
- Zebrafish/anatomy & histology
- PubMed
- 24979011 Full text @ Opt. Lett.
Citation
Poland, S.P., Krstajic, N., Knight, R.D., Henderson, R.K., Ameer-Beg, S.M. (2014) Development of a doubly weighted Gerchberg-Saxton algorithm for use in multibeam imaging applications. Optics letters. 39:2431-2434.
Abstract
We report on the development of a doubly weighted Gerchberg-Saxton algorithm (DWGS) to enable generation of uniform beamlet arrays with a spatial light modulator (SLM) for use in multiphoton multifocal imaging applications. The algorithm incorporates the WGS algorithm as well as feedback of fluorescence signals from the sample measured with a single-photon avalanche diode (SPAD) detector array. This technique compensates for issues associated with nonuniform illumination onto the SLM, the effects due to aberrations and the variability in gain between detectors within the SPAD array to generate a uniformly illuminated multiphoton fluorescence image. We demonstrate the use of the DWGS with a number of beamlet array patterns to image muscle fibers of a 5-day-old fixed zebrafish larvae.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping