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ZFIN ID: ZDB-PUB-140621-11
The zebrafish/tumor xenograft angiogenesis assay as a tool for screening anti-angiogenic miRNAs
Chiavacci, E., Rizzo, M., Pitto, L., Patella, F., Evangelista, M., Mariani, L., Rainaldi, G.
Date: 2015
Source: Cytotechnology 67(6): 969-75 (Journal)
Registered Authors: Chiavacci, Elena
Keywords: microRNAs, Tumor angiogenesis, Zebrafish/tumor xenografts, Prostate tumor cells
MeSH Terms: none
PubMed: 24947063 Full text @ Cytotechnology
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ABSTRACT
The zebrafish/tumor xenograft angiogenesis assay is used to approach tumor angiogenesis, a pivotal step in cancer progression and target for anti-tumor therapies. Here, we evaluated whether the assay could allow the identification of microRNAs having an anti-angiogenic potential. For that, we transfected DU-145 prostate cancer cells with four microRNAs (miR-125a, miR-320, miR-487b, miR-492) responsive to both anti- and pro-angiogenic stimuli applied to human umbilical vein endothelial cells. After transfection, DU-145 cells were injected close to the developing subintestinal vessels of transgenic Tg(Kdrl:eGFP)s843 zebrafish embryos that express green fluorescent protein under the control of Kdrl promoter. At 72 h post-fertilization, we observed that green fluorescent protein-positive neo-vessels infiltrated the graft of DU-145 transfected with miR-125a, miR-320, and miR-487b. Vice versa, neo-vessel formation and tumor cell infiltration were inhibited when DU-145 cells transfected with miR-492 were used. These results indicated that the zebrafish/tumor xenograft assay was adequate to identify microRNAs able to suppress the release of angiogenic growth factors by angiogenic tumor cells.
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