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ZFIN ID: ZDB-PUB-140521-2
ATP Modulates Acute Inflammation In Vivo through Dual Oxidase 1-Derived H2O2 Production and NF-kappaB Activation
de Oliveira, S., López-Muñoz, A., Candel, S., Pelegrín, P., Calado, A., Mulero, V.
Date: 2014
Source: Journal of immunology (Baltimore, Md. : 1950)   192(12): 5710-9 (Journal)
Registered Authors: de Oliveira, Sofia, Mulero, Victor
Keywords: none
MeSH Terms:
  • Acute Disease
  • Adenosine Triphosphate/immunology*
  • Animals
  • Calcium Signaling/immunology*
  • Hydrogen Peroxide/immunology*
  • Inflammation
  • NADPH Oxidases/immunology*
  • NF-kappa B/immunology*
  • Receptors, Purinergic P2Y/immunology
  • Wounds and Injuries/immunology*
  • Zebrafish
  • Zebrafish Proteins/immunology*
PubMed: 24842759 Full text @ J. Immunol.
Dual oxidase 1 (Duox1) is the NADPH oxidase responsible for the H2O2 gradient formed in tissues after injury to trigger the early recruitment of leukocytes. Little is known about the signals that modulate H2O2 release from DUOX1 and whether the H2O2 gradient can orchestrate the inflammatory response in vivo. In this study, we report on a dominant-negative form of zebrafish Duox1 that is able to inhibit endogenous Duox1 activity, H2O2 release and leukocyte recruitment after tissue injury, with none of the side effects associated with morpholino-mediated Duox1 knockdown. Using this specific tool, we found that ATP release following tissue injury activates purinergic P2Y receptors, and modulates Duox1 activity through phospholipase C (PLC) and intracellular calcium signaling in vivo. Furthermore, Duox1-derived H2O2 is able to trigger the NF-κB inflammatory signaling pathway. These data reveal that extracellular ATP acting as an early danger signal is responsible for the activation of Duox1 via a P2YR/PLC/Ca(2+) signaling pathway and the production of H2O2, which, in turn, is able to modulate in vivo not only the early recruitment of leukocytes to the wound but also the inflammatory response through activation of the NF-κB signaling pathway.