ZFIN ID: ZDB-PERS-090406-5
Mulero, Victor
Email: vmulero@um.es
URL: http://www.um.es/nisoft/victor1.htm
Affiliation: Mulero Lab
Address: Department of Cell Biology and Histology Faculty of Biology University of Murcia Campus Universitario de Espinardo Murcia, 30100 Spain
Country: Spain
Phone: +34868887581
Fax: +34868883963
ORCID ID: 0000-0001-9527-0211

Our laboratory is interested in the evolution of innate immunity. We work with several fish species with great commercial importance, such us the gilthead seabream and the European seabass, as well as the zebrafish. Our main research lines include the functional characterisation of fish phagocytes and mast cells, the characterization of TLRs, in particular TLR4 and soluble TLR5, the biological activities of cytokines (IL-1beta, TNFalpha and IFNs), and the ontogeny of immunity for the design of proper vaccination schedules for aquaculture. The laboratory has developed during the last years several antibodies specific to seabream phagocytes and cytokines that constitute valuable tools for investigating the immune response of this commercial important species.

Arroyo, A.B., Bernal-Carrión, M., Cantón-Sandoval, J., Cabas, I., Corbalán-Vélez, R., Martínez-Menchón, T., Ferri, B., Cayuela, M.L., García-Moreno, D., Mulero, V. (2023) NAMPT and PARylation Are Involved in the Pathogenesis of Atopic Dermatitis. International Journal of Molecular Sciences. 24(9):
Pardo-Sánchez, I., Ibañez-Molero, S., García-Moreno, D., Mulero, V. (2023) Dual Role of DUOX1-Derived Reactive Oxygen Species in Melanoma. Antioxidants (Basel, Switzerland). 12(3):
Tyrkalska, S.D., Martínez-López, A., Pedoto, A., Candel, S., Cayuela, M.L., Mulero, V. (2022) The Spike protein of SARS-CoV-2 signals via Tlr2 in zebrafish. Developmental and comparative immunology. 140:104626
Tyrkalska, S.D., Martínez-López, A., Arroyo, A.B., Martínez-Morcillo, F.J., Candel, S., García-Moreno, D., Mesa-Del-Castillo, P., Cayuela, M.L., Mulero, V. (2022) Differential proinflammatory activities of Spike proteins of SARS-CoV-2 variants of concern. Science advances. 8:eabo0732
Hernández-Silva, D., Cantón-Sandoval, J., Martínez-Navarro, F.J., Pérez-Sánchez, H., de Oliveira, S., Mulero, V., Alcaraz-Pérez, F., Cayuela, M.L. (2022) Senescence-Independent Anti-Inflammatory Activity of the Senolytic Drugs Dasatinib, Navitoclax, and Venetoclax in Zebrafish Models of Chronic Inflammation. International Journal of Molecular Sciences. 23(18)
Tyrkalska, S.D., Pedoto, A., Martínez-López, A., Ros-Lucas, J.A., Mesa-Del-Castillo, P., Candel, S., Mulero, V. (2022) Silica crystals activate toll-like receptors and inflammasomes to promote local and systemic immune responses in zebrafish. Developmental and comparative immunology. 138:104523
Fatás-Lalana, B., Cantón-Sandoval, J., Rodríguez-Ruiz, L., Corbalán-Vélez, R., Martínez-Menchón, T., Pérez-Oliva, A.B., Mulero, V. (2022) Impact of Comorbidities of Patients with Psoriasis on Phototherapy Responses. International Journal of Molecular Sciences. 23(17)
Di Chiacchio, I.M., Gómez-Abenza, E., Paiva, I.M., de Abreu, D.J.M., Rodríguez-Vidal, J.F., Carvalho, E.E.N., Carvalho, S.M., Solis-Murgas, L.D., Mulero, V. (2022) Bee pollen in zebrafish diet affects intestinal microbiota composition and skin cutaneous melanoma development. Scientific Reports. 12:9998
Lozano-Gil, J.M., Rodríguez-Ruiz, L., Tyrkalska, S.D., García-Moreno, D., Pérez-Oliva, A.B., Mulero, V. (2022) Gasdermin E mediates pyroptotic cell death of neutrophils and macrophages in a zebrafish model of chronic skin inflammation. Developmental and comparative immunology. 132:104404
Martínez-Morcillo, F.J., Cantón-Sandoval, J., Martínez-Navarro, F.J., Cabas, I., Martínez-Vicente, I., Armistead, J., Hatzold, J., López-Muñoz, A., Martínez-Menchón, T., Corbalán-Vélez, R., Lacal, J., Hammerschmidt, M., García-Borrón, J.C., García-Ayala, A., Cayuela, M.L., Pérez-Oliva, A.B., García-Moreno, D., Mulero, V. (2021) NAMPT-derived NAD+ fuels PARP1 to promote skin inflammation through parthanatos cell death. PLoS Biology. 19:e3001455
Di Chiacchio, I.M., Paiva, I.M., de Abreu, D.J.M., Carvalho, E.E.N., Martínez, P.J., Carvalho, S.M., Mulero, V., Murgas, L.D.S. (2021) Bee pollen as a dietary supplement for fish: Effect on the reproductive performance of zebrafish and the immunological response of their offspring. Fish & shellfish immunology. 119:300-307
Lee, L., Perez Oliva, A.B., Martinez-Balsalobre, E., Churikov, D., Peter, J., Rahmouni, D., Audoly, G., Azzoni, V., Audebert, S., Camoin, L., Mulero, V., Cayuela, M.L., Kulathu, Y., Geli, V., Lachaud, C. (2021) UFMylation of MRE11 is essential for telomere length maintenance and hematopoietic stem cell survival. Science advances. 7:eabc7371
García-Castillo, J., Alcaraz-Pérez, F., Martínez-Balsalobre, E., García-Moreno, D., Rossmann, M.P., Fernández-Lajarín, M., Bernabé-García, M., Pérez-Oliva, A.B., Rodríguez-Cortez, V.C., Bueno, C., Adatto, I., Agarwal, S., Menéndez, P., Zon, L.I., Mulero, V., Cayuela, M.L. (2021) Telomerase RNA recruits RNA polymerase II to target gene promoters to enhance myelopoiesis. Proceedings of the National Academy of Sciences of the United States of America. 118(32):
Rodríguez-Ruiz, L., Lozano-Gil, J.M., Lachaud, C., Mesa-Del-Castillo, P., Cayuela, M.L., García-Moreno, D., Pérez-Oliva, A.B., Mulero, V. (2020) Zebrafish Models to Study Inflammasome-Mediated Regulation of Hematopoiesis. Trends in immunology. 41(12):1116-1127
Tyrkalska, S.D., Candel, S., Mulero, V. (2020) The neutrophil inflammasome. Developmental and comparative immunology. 115:103874
Camiolo, G., Barbato, A., Giallongo, C., Vicario, N., Romano, A., Parrinello, N.L., Parenti, R., Sandoval, J.C., García-Moreno, D., Lazzarino, G., Avola, R., Palumbo, G.A., Mulero, V., Li Volti, G., Tibullo, D., Di Raimondo, F. (2020) Iron regulates myeloma cell/macrophage interaction and drives resistance to bortezomib. Redox Biology. 36:101611
Siriyappagouder, P., Galindo-Villegas, J., Dhanasiri, A.K.S., Zhang, Q., Mulero, V., Kiron,V., Fernandes, J.M.O. (2020) Pseudozyma Priming Influences Expression of Genes Involved in Metabolic Pathways and Immunity in Zebrafish Larvae. Frontiers in immunology. 11:978
Lopez-Guerrero, A.M., Espinosa-Bermejo, N., Sanchez-Lopez, I., Macartney, T., Pascual-Caro, C., Orantos-Aguilera, Y., Rodriguez-Ruiz, L., Perez-Oliva, A.B., Mulero, V., Pozo-Guisado, E., Martin-Romero, F.J. (2020) RAC1-Dependent ORAI1 Translocation to the Leading Edge Supports Lamellipodia Formation and Directional Persistence. Scientific Reports. 10:6580
Martínez-Navarro, F.J., Martínez-Morcillo, F.J., López-Muñoz, A., Pardo-Sánchez, I., Martínez-Menchón, T., Corbalán-Vélez, R., Cayuela, M.L., Pérez-Oliva, A.B., García-Moreno, D., Mulero, V. (2020) The vitamin B6-dependent enzymes PYGL and G6PD fuel NADPH oxidases to promote skin inflammation. Developmental and comparative immunology. 108:103666
Martínez-Navarro, F.J., Martínez-Morcillo, F.J., de Oliveira, S., Candel, S., Cabas, I., García-Ayala, A., Martínez-Menchón, T., Corbalán-Vélez, R., Mesa-Del-Castillo, P., Cayuela, M.L., Pérez-Oliva, A.B., García-Moreno, D., Mulero, V. (2019) Hydrogen peroxide in neutrophil inflammation: Lesson from the zebrafish. Developmental and comparative immunology. 105:103583
Gómez-Abenza, E., Ibáñez-Molero, S., García-Moreno, D., Fuentes, I., Zon, L.I., Mione, M.C., Cayuela, M.L., Gabellini, C., Mulero, V. (2019) Zebrafish modeling reveals that SPINT1 regulates the aggressiveness of skin cutaneous melanoma and its crosstalk with tumor immune microenvironment. Journal of experimental & clinical cancer research : CR. 38:405
Novoa, B., Pereiro, P., López-Muñoz, A., Varela, M., Forn-Cuní, G., Anchelin, M., Dios, S., Romero, A., Martinez-López, A., Medina-Gali, R.M., Collado, M., Coll, J., Estepa, A., Cayuela, M.L., Mulero, V., Figueras, A. (2019) Rag1 immunodeficiency-induced early aging and senescence in zebrafish are dependent on chronic inflammation and oxidative stress. Aging Cell. 18(5):e13020
Sepahi, A., Kraus, A., Casadei, E., Johnston, C.A., Galindo-Villegas, J., Kelly, C., García-Moreno, D., Muñoz, P., Mulero, V., Huertas, M., Salinas, I. (2019) Olfactory sensory neurons mediate ultrarapid antiviral immune responses in a TrkA-dependent manner. Proceedings of the National Academy of Sciences of the United States of America. 116(25):12428-12436
Tyrkalska, S.D., Pérez-Oliva, A.B., Rodríguez-Ruiz, L., Martínez-Morcillo, F.J., Alcaraz-Pérez, F., Martínez-Navarro, F.J., Lachaud, C., Ahmed, N., Schroeder, T., Pardo-Sánchez, I., Candel, S., López-Muñoz, A., Choudhuri, A., Rossmann, M.P., Zon, L.I., Cayuela, M.L., García-Moreno, D., Mulero, V. (2019) Inflammasome Regulates Hematopoiesis through Cleavage of the Master Erythroid Transcription Factor GATA1. Immunity. 51(1):50-63.e5
García-Moreno, D., Tyrkalska, S.D., Valera-Pérez, A., Gómez-Abenza, E., Pérez Oliva, A.B., Mulero, V. (2019) The Zebrafish: A Research Model To Understand The Evolution Of Vertebrate Immunity. Fish & shellfish immunology. 90:215-222
Martínez-Navarro, F.J., Martínez-Menchón, T., Mulero, V., Galindo-Villegas, J. (2019) Models of human psoriasis: Zebrafish the newly appointed player. Developmental and comparative immunology. 97:76-87
López-Muñoz, A., Nicolás, F.E., García-Moreno, D., Pérez-Oliva, A.B., Navarro-Mendoza, M.I., Hernández-Oñate, M.A., Herrera-Estrella, A., Torres-Martínez, S., Ruiz-Vázquez, R.M., Garre, V., Mulero, V. (2018) An Adult Zebrafish Model Reveals that Mucormycosis Induces Apoptosis of Infected Macrophages. Scientific Reports. 8:12802
Siriyappagouder, P., Galindo-Villegas, J., Lokesh, J., Mulero, V., Fernandes, J., and Kiron, V. (2018) Exposure to Yeast Shapes the Intestinal Bacterial Community Assembly in Zebrafish Larvae. Frontiers in microbiology. 9:1868
Tyrkalska, S.D., Candel, S., Pérez-Oliva, A.B., Valera, A., Alcaraz-Pérez, F., García-Moreno, D., Cayuela, M.L., Mulero, V. (2017) Identification of an Evolutionarily Conserved Ankyrin Domain-Containing Protein, Caiap, Which Regulates Inflammasome-Dependent Resistance to Bacterial Infection. Frontiers in immunology. 8:1375
Gabellini, C., Gómez-Abenza, E., Ibáñez-Molero, S., Tupone, M.G., Pérez-Oliva, A.B., de Oliveira, S., Del Bufalo, D., Mulero, V. (2017) Interleukin 8 mediates bcl-xL-induced enhancement of human melanoma cell dissemination and angiogenesis in a zebrafish xenograft model. International Journal of Cancer. 142(3):584-596
García-Valtanen, P., Martínez-López, A., López-Muñoz, A., Bello-Perez, M., Medina-Gali, R.M., Ortega-Villaizán, M.D., Varela, M., Figueras, A., Mulero, V., Novoa, B., Estepa, A., Coll, J. (2017) Zebra Fish Lacking Adaptive Immunity Acquire an Antiviral Alert State Characterized by Upregulated Gene Expression of Apoptosis, Multigene Families, and Interferon-Related Genes. Frontiers in immunology. 8:121
Candel, S., Tyrkalska, S.D., García-Moreno, D., Meseguer, J., Mulero, V. (2016) Identification of Evolutionarily Conserved Md1 Splice Variants That Regulate Innate Immunity through Differential Induction of NF-кB. Journal of immunology (Baltimore, Md. : 1950). 197(4):1379-88
Tyrkalska, S.D., Candel, S., Angosto, D., Gómez-Abellán, V., Martín-Sánchez, F., García-Moreno, D., Zapata-Pérez, R., Sánchez-Ferrer, Á., Sepulcre, M.P., Pelegrín, P., Mulero, V. (2016) Neutrophils mediate Salmonella Typhimurium clearance through the GBP4 inflammasome-dependent production of prostaglandins. Nature communications. 7:12077
Espín-Palazón, R., Martínez-López, A., Roca, F.J., López-Muñoz, A., Tyrkalska, S.D., Candel, S., García-Moreno, D., Falco, A., Meseguer, J., Estepa, A., Mulero, V. (2016) TNFα Impairs Rhabdoviral Clearance by Inhibiting the Host Autophagic Antiviral Response. PLoS pathogens. 12:e1005699
Galindo-Villegas, J., Montalban-Arques, A., Liarte, S., de Oliveira, S., Pardo-Pastor, C., Rubio-Moscardo, F., Meseguer, J., Valverde, M.A., Mulero, V. (2016) TRPV4-Mediated Detection of Hyposmotic Stress by Skin Keratinocytes Activates Developmental Immunity. Journal of immunology (Baltimore, Md. : 1950). 196(2):738-49
Rodenas, M.C., Cabas, I., Abellán, E., Meseguer, J., Mulero, V., García-Ayala, A. (2015) Tamoxifen persistently disrupts the humoral adaptive immune response of gilthead seabream (Sparus aurata L.). Developmental and comparative immunology. 53:283-92
López-Muñoz, A., Liarte, S., Gómez-González, N.E., Cabas, I., Meseguer, J., García-Ayala, A., Mulero, V. (2015) Estrogen receptor 2b deficiency impairs the antiviral response of zebrafish. Developmental and comparative immunology. 53(1):55-62
Candel, S., Sepulcre, M.P., Espín-Palazón, R., Tyrkalska, S.D., de Oliveira, S., Meseguer, J., Mulero, V. (2015) Md1 and Rp105 regulate innate immunity and viral resistance in zebrafish. Developmental and comparative immunology. 50(2):155-65
de Oliveira, S., Lopez-Muñoz, A., Martínez-Navarro, F.J., Galindo-Villegas, J., Mulero, V., Calado, A. (2015) Cxcl8-l1 and Cxcl8-l2 are required in the zebrafish defense against Salmonella Typhimurium. Developmental and comparative immunology. 49:44-48
de Oliveira, S., Boudinot, P., Calado, Â., Mulero, V. (2015) Duox1-Derived H2O2 Modulates Cxcl8 Expression and Neutrophil Recruitment via JNK/c-JUN/AP-1 Signaling and Chromatin Modifications. Journal of immunology (Baltimore, Md. : 1950). 194(4):1523-33
Espín-Palazón, R., Stachura, D.L., Campbell, C.A., García-Moreno, D., Del Cid, N., Kim, A.D., Candel, S., Meseguer, J., Mulero, V., Traver, D. (2014) Proinflammatory signaling regulates hematopoietic stem cell emergence. Cell. 159:1070-85
de Oliveira, S., López-Muñoz, A., Candel, S., Pelegrín, P., Calado, A., Mulero, V. (2014) ATP Modulates Acute Inflammation In Vivo through Dual Oxidase 1-Derived H2O2 Production and NF-kappaB Activation. Journal of immunology (Baltimore, Md. : 1950). 192(12):5710-9
Candel, S., de Oliveira, S., López-Muñoz, A., García-Moreno, D., Espín-Palazón, R., Tyrkalska, S.D., Cayuela, M.L., Renshaw, S.A., Corbalán-Vélez, R., Vidal-Abarca, I., Tsai, H.J., Meseguer, J., Sepulcre, M.P., Mulero, V. (2014) Tnfa signaling through tnfr2 protects skin against oxidative stress-induced inflammation. PLoS Biology. 12:e1001855
Alcaraz-Pérez, F., García-Castillo, J., García-Moreno, D., López-Muñoz, A., Anchelin, M., Angosto, D., Zon, L.I., Mulero, V., and Cayuela, M.L. (2014) A non-canonical function of telomerase RNA in the regulation of developmental myelopoiesis in zebrafish. Nature communications. 5:3228
Anchelin, M., Alcaraz-Pérez, F., Martínez, C.M., Bernabé-García, M., Mulero, V., and Cayuela, M.L. (2013) Premature aging in telomerase-deficient zebrafish. Disease models & mechanisms. 6(5):1101-12
de Oliveira, S., Reyes-Aldasoro, C.C., Candel, S., Renshaw, S.A., Mulero, V., and Calado, A. (2013) Cxcl8 (IL-8) Mediates Neutrophil Recruitment and Behavior in the Zebrafish Inflammatory Response. Journal of immunology (Baltimore, Md. : 1950). 190(8):4349-59
Espín, R., Roca, F.J., Candel, S., Sepulcre, M.P., González-Rosa, J.M., Alcaraz-Pérez, F., Meseguer, J., Cayuela, M.L., Mercader, N., and Mulero, V. (2013) TNF receptors regulate vascular homeostasis through a caspase-8, caspase-2 and P53 apoptotic program that bypasses caspase-3. Disease models & mechanisms. 6(2):383-396
Galindo-Villegas, J., García-Moreno, D., de Oliveira, S., Meseguer, J., and Mulero, V. (2012) Regulation of immunity and disease resistance by commensal microbes and chromatin modifications during zebrafish development. Proceedings of the National Academy of Sciences of the United States of America. 109(39):E2605-2614
López-Muñoz, A., Sepulcre, M.P., Roca, F.J., Figueras, A., Meseguer, J., and Mulero, V. (2011) Evolutionary conserved pro-inflammatory and antigen presentation functions of zebrafish IFNγ revealed by transcriptomic and functional analysis. Molecular immunology. 48(9-10):1073-1083
López-Muñoz, A., Roca, F.J., Sepulcre, M.P., Meseguer, J., and Mulero, V. (2010) Zebrafish Larvae Are Unable To Mount a Protective Antiviral Response Against Waterborne Infection by Spring Viremia of Carp Virus. Developmental and comparative immunology. 34(5):546-552
López-Muñoz, A., Roca, F.J., Meseguer, J., and Mulero, V. (2009) New insights into the evolution of IFNs: zebrafish Group II IFNs induce a rapid and transient expression of IFN-dependent genes and display powerful antiviral activities. Journal of immunology (Baltimore, Md. : 1950). 182(6):3440-3449
Sepulcre, M.P., Alcaraz-Pérez, F., López-Muñoz, A., Roca, F.J., Meseguer, J., Cayuela, M.L., and Mulero, V. (2009) Evolution of lipopolysaccharide (LPS) recognition and signaling: fish TLR4 does not recognize LPS and negatively regulates NF-kappaB activation. Journal of immunology (Baltimore, Md. : 1950). 182(4):1836-1845
Alcaraz-Perez, F., Mulero, V., and Cayuela, M.L. (2008) Application of the dual-luciferase reporter assay to the analysis of promoter activity in zebrafish embryos. BMC Biotechnology. 8:81
Roca, F.J., Mulero, I., López-Muñoz, A., Sepulcre, M.P., Renshaw, S.A., Meseguer, J., and Mulero, V. (2008) Evolution of the Inflammatory Response in Vertebrates: Fish TNF-{alpha} Is a Powerful Activator of Endothelial Cells but Hardly Activates Phagocytes. Journal of immunology (Baltimore, Md. : 1950). 181(7):5071-5081
Mulero, I., Sepulcre, M.P., Meseguer, J., García-Ayala, A., and Mulero, V. (2007) Histamine is stored in mast cells of most evolutionarily advanced fish and regulates the fish inflammatory response. Proceedings of the National Academy of Sciences of the United States of America. 104(49):19434-19439