PUBLICATION

Simultaneous Raman microspectroscopy and fluorescence imaging of bone mineralization in living zebrafish larvae

Authors
Bennet, M., Akiva, A., Faivre, D., Malkinson, G., Yaniv, K., Abdelilah-Seyfried, S., Fratzl, P., Masic, A.
ID
ZDB-PUB-140513-491
Date
2014
Source
Biophysical journal   106: L17-9 (Journal)
Registered Authors
Abdelilah-Seyfried, Salim, Malkinson, Guy, Yaniv, Karina
Keywords
none
MeSH Terms
  • Animals
  • Calcification, Physiologic*
  • Larva/physiology
  • Microscopy, Fluorescence/methods*
  • Optical Imaging/methods*
  • Spectrum Analysis, Raman/methods*
  • Zebrafish
PubMed
24560001 Full text @ Biophys. J.
Abstract
Confocal Raman microspectroscopy and fluorescence imaging are two well-established methods providing functional insight into the extracellular matrix and into living cells and tissues, respectively, down to single molecule detection. In living tissues, however, cells and extracellular matrix coexist and interact. To acquire information on this cell-matrix interaction, we developed a technique for colocalized, correlative multispectral tissue analysis by implementing high-sensitivity, wide-field fluorescence imaging on a confocal Raman microscope. As a proof of principle, we study early stages of bone formation in the zebrafish (Danio rerio) larvae because the zebrafish has emerged as a model organism to study vertebrate development. The newly formed bones were stained using a calcium fluorescent marker and the maturation process was imaged and chemically characterized in vivo. Results obtained from early stages of mineral deposition in the zebrafish fin bone unequivocally show the presence of hydrogen phosphate containing mineral phases in addition to the carbonated apatite mineral. The approach developed here opens significant opportunities in molecular imaging of metabolic activities, intracellular sensing, and trafficking as well as in vivo exploration of cell-tissue interfaces under (patho-)physiological conditions.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes