PUBLICATION
TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella
- Authors
- Ishikawa, H., Ide, T., Yagi, T., Jiang, X., Hirono, M., Sasaki, H., Yanagisawa, H., Wemmer, K.A., Stainier, D.Y., Qin, H., Kamiya, R., Marshall, W.F.
- ID
- ZDB-PUB-140513-410
- Date
- 2014
- Source
- eLIFE 3: e01566 (Journal)
- Registered Authors
- Stainier, Didier
- Keywords
- Chlamydomonas, axoneme, dynein, flagella
- MeSH Terms
-
- Cilia/metabolism*
- Chlamydomonas reinhardtii/genetics
- Chlamydomonas reinhardtii/metabolism*
- Flagella/metabolism*
- Plant Proteins/genetics
- Plant Proteins/metabolism*
- Transfection
- Embryo, Nonmammalian/metabolism
- Cell Movement*
- Algal Proteins/genetics
- Algal Proteins/metabolism*
- Intracellular Signaling Peptides and Proteins/genetics
- Intracellular Signaling Peptides and Proteins/metabolism*
- Mice
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Carrier Proteins/genetics
- Carrier Proteins/metabolism*
- Gene Expression Regulation, Developmental
- Phenotype
- Animals
- Genotype
- Cell Line
- Gene Knockdown Techniques
- Mutation
- Protein Transport
- PubMed
- 24596149 Full text @ Elife
Citation
Ishikawa, H., Ide, T., Yagi, T., Jiang, X., Hirono, M., Sasaki, H., Yanagisawa, H., Wemmer, K.A., Stainier, D.Y., Qin, H., Kamiya, R., Marshall, W.F. (2014) TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella. eLIFE. 3:e01566.
Abstract
Cilia/flagella are assembled and maintained by the process of intraflagellar transport (IFT), a highly conserved mechanism involving more than 20 IFT proteins. However, the functions of individual IFT proteins are mostly unclear. To help address this issue, we focused on a putative IFT protein TTC26/DYF13. Using live imaging and biochemical approaches we show that TTC26/DYF13 is an IFT complex B protein in mammalian cells and Chlamydomonas reinhardtii. Knockdown of TTC26/DYF13 in zebrafish embryos or mutation of TTC26/DYF13 in C. reinhardtii, produced short cilia with abnormal motility. Surprisingly, IFT particle assembly and speed were normal in dyf13 mutant flagella, unlike in other IFT complex B mutants. Proteomic and biochemical analyses indicated a particular set of proteins involved in motility was specifically depleted in the dyf13 mutant. These results support the concept that different IFT proteins are responsible for different cargo subsets, providing a possible explanation for the complexity of the IFT machinery.
Errata / Notes
This article is corrected by ZDB-PUB-220906-8.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping