PUBLICATION
The Comparison of Methods for Measuring Oxidative Stress in Zebrafish Brains
- Authors
- Moussavi Nik, S.H., Croft, K., Mori, T.A., Lardelli, M.
- ID
- ZDB-PUB-140513-35
- Date
- 2014
- Source
- Zebrafish 11(3): 248-54 (Journal)
- Registered Authors
- Lardelli, Michael
- Keywords
- none
- MeSH Terms
-
- Animals
- Brain/metabolism*
- Catalase/genetics
- Catalase/metabolism
- F2-Isoprostanes/metabolism
- Female
- Fluoresceins/metabolism
- Gas Chromatography-Mass Spectrometry/methods*
- Gene Expression
- Lipid Peroxidation*
- Lipid Peroxides/metabolism
- Male
- Neuroprostanes/metabolism
- Oxidative Stress*
- Reactive Oxygen Species/metabolism
- Real-Time Polymerase Chain Reaction/methods*
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 24798242 Full text @ Zebrafish
Citation
Moussavi Nik, S.H., Croft, K., Mori, T.A., Lardelli, M. (2014) The Comparison of Methods for Measuring Oxidative Stress in Zebrafish Brains. Zebrafish. 11(3):248-54.
Abstract
Abstract The zebrafish is a versatile model organism with the potential to contribute to our understanding of the molecular pathological mechanisms underlying Alzheimer's disease (AD). An early characteristic of AD brain pathology is lipid peroxidation resulting from oxidative stress. However, changes in lipid peroxidation have not yet been assessed in zebrafish brains, and an earlier attempt to observe changes in F2-isoprostane levels in the brains of zebrafish exposed to hypoxia was unsuccessful. In this article, we examine the utility of various assays of lipid peroxidation and more general assays of intracellular oxidative stress to detect the changes in oxidative stress in the brains of adult zebrafish exposed to hypoxia or explanted into a sodium azide solution for chemical mimicry of hypoxia. Levels of F2-isoprostanes and F4-neuroprostanes were low and variable in zebrafish brains such that statistically significant changes due to hypoxia or chemical mimicry of hypoxia could not be observed. However, measurement of lipid hydroperoxides did reveal significant changes in lipid peroxidation under these conditions, while analyses of catalase gene expression and an assay based on 2',7'-dicholorofluorescein oxidation also revealed changes in oxidative stress levels.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping