ZFIN ID: ZDB-PERS-960805-318
Lardelli, Michael
Email: michael.lardelli@adelaide.edu.au
URL: http://biological.adelaide.edu.au/research/zege/
Affiliation: Lardelli Lab
Address: Alzheimer's Disease Genetics Laboratory Centre for Molecular Pathology School of Biological Sciences University of Adelaide North Terrace SA 5005 Australia
Country: Australia
Phone: 61-8-8313-3212
Fax:
ORCID ID: 0000-0002-4289-444X


BIOGRAPHY AND RESEARCH INTERESTS
ACADEMIC BACKGROUND

1984, Bachelor of Science (Honours class 1, Biology - Genetics),
University of Sydney,
Australia.

March 1991, Ph.D.
Council for National Academic Awards,
United Kingdom.


RESEARCH EXPERIENCE

1984 (February-November) Honours Degree Candidate, Department of Biology, University of Sydney, Australia. Laboratory of Dr. Keith D. Brown. Regulation of K99pilin'-lac'Z gene fusions in Escherichia coli.

1985 (January-December), Research Assistant, Garvan Institute of Medical Research, Sydney, Australia. Laboratory of Dr. John Eisman. Effects of 1,25 (OH)2 vitamin D3 on breast cancer cells.

1985 (December) - 1986 (May), Research Assistant, Department of Microbiology, University of Sydney, Australia. Laboratory of Prof. Peter Reeves. Development of a human rotavirus vaccine.

1987 (April-August), Biochemistry Department, University of Oxford, U.K.. Assistant to Dr. William Brown in laboratory of Prof. Edwin Southern. Mapping of the human Y-chromosome.

1987 (September) to 1991 (March), PhD studies - Imperial Cancer Research Fund Developmental Biology Unit, Oxford, U.K.. Laboratory of Dr. David Ish-Horowicz. Regulation and function of the Drosophila pair-rule gene hairy.

1991 (March) to 1994 (July), Postdoctoral studies - The Karolinska Institute, Stockholm, Sweden. Laboratory of Dr. Urban Lendahl. Molecular genetic analysis of early mouse neural development.

1994 (July to August), Laboratory of Philip Ingham, ICRF, London, U.K.. Expression of a mouse Notch3 transgene in the zebrafish.

1994 (July) to 1995 (June), Postdoctoral studies at Uppsala University Biomedical Center, Sweden. Department of Developmental Neuroscience. Genetic studies of vertebrate development using the zebrafish.

1995 (July) to 1997 (May), Assistant Professor at Uppsala University Biomedical Center, Sweden. Department of Developmental Neuroscience. Genetic studies of vertebrate development using the zebrafish.

1997 (June onwards), Lecturer in Genetics (tenured since 2000) in the Department of Molecular Bioscience of The University of Adelaide, Australia. Genetic studies of vertebrate development using the zebrafish.

2003 onwards, Senior Lecturer in Genetics in the Department of Molecular Bioscience of The University of Adelaide, Australia. Genetic studies of vertebrate development using the zebrafish.

2015 onwards, Associate Professor in Genetics, Alzheimer's Disease Genetics Laboratory, Centre for Molecular Pathology, School of Biological Sciences, The University of Adelaide, Australia. Investigating the molecular basis of Alzheimer's disease using zebrafish.


CURRENT RESEARCH INTERESTS

- Investigation of the molecular basis of Alzheimer's disease

- Presenilin genes, APP, Notch signalling


PUBLICATIONS
Barthelson, K., Pederson, S.M., Newman, M., Lardelli, M. (2020) Brain transcriptome analysis reveals subtle effects on mitochondrial function and iron homeostasis of mutations in the SORL1 gene implicated in early onset familial Alzheimer's disease. Molecular brain. 13:142
Jiang, H., Pederson, S.M., Newman, M., Dong, Y., Barthelson, K., Lardelli, M. (2020) Transcriptome analysis indicates dominant effects on ribosome and mitochondrial function of a premature termination codon mutation in the zebrafish gene psen2. PLoS One. 15:e0232559
Hin, N., Newman, M., Kaslin, J., Douek, A.M., Lumsden, A., Nik, S.H.M., Dong, Y., Zhou, X.F., Mañucat-Tan, N.B., Ludington, A., Adelson, D.L., Pederson, S., Lardelli, M. (2020) Accelerated brain aging towards transcriptional inversion in a zebrafish model of the K115fs mutation of human PSEN2. PLoS One. 15:e0227258
Newman, M., Hin, N., Pederson, S., Lardelli, M. (2019) Brain transcriptome analysis of a familial Alzheimer's disease-like mutation in the zebrafish presenilin 1 gene implies effects on energy production. Molecular brain. 12:43
Jiang, H., Newman, M., Lardelli, M. (2018) The zebrafish orthologue of familial Alzheimer's disease gene PRESENILIN 2 is required for normal adult melanotic skin pigmentation. PLoS One. 13:e0206155
Ratnayake, D., Newman, M., Lardelli, M. (2018) Degenerate codon mixing for PCR-based manipulation of highly repetitive sequences. BMC research notes. 11:202
Newman, M., Halter, L., Lim, A., Lardelli, M. (2017) Mitochondrion to endoplasmic reticulum apposition length in zebrafish embryo spinal progenitors is unchanged in response to perturbations associated with Alzheimer's disease. PLoS One. 12:e0179859
Ebrahimie, E., Moussavi-Nik, S.H., Newman, M., Van Der Hoek, M., Lardelli, M. (2016) The Zebrafish Equivalent of Alzheimer's Disease-Associated PRESENILIN Isoform PS2V Regulates Inflammatory and Other Responses to Hypoxic Stress. Journal of Alzheimer's disease : JAD. 52(2):581-608
Moussavi-Nik, S.H., Newman, M., Wilson, L., Ebrahimie, E., Wells, S., Musgrave, I., Verdile, G., Martins, R.N., Lardelli, M. (2015) Alzheimer's disease-related peptide PS2V plays ancient, conserved roles in suppression of the unfolded protein response under hypoxia and stimulation of γ-secretase activity. Human molecular genetics. 24(13):3662-78
Lim, A., Moussavi Nik, S.H., Ebrahimie, E., Lardelli, M. (2015) Analysis of nicastrin gene phylogeny and expression in zebrafish. Development genes and evolution. 225(3):171-8
Klarić, T., Lardelli, M., Key, B., Koblar, S., Lewis, M. (2014) Activity-dependent expression of neuronal PAS domain-containing protein 4 (npas4a) in the developing zebrafish brain. Frontiers in Neuroanatomy. 8:148
Moussavi Nik, S.H., Newman, M., Ganesan, S., Chen, M., Martins, R., Verdile, G., Lardelli, M. (2014) Hypoxia alters expression of Zebrafish Microtubule-associated protein Tau (mapta, maptb) gene transcripts. BMC research notes. 7:767
Ganesan, S., Moussavi Nik, S.H., Newman, M., Lardelli, M. (2014) Identification and expression analysis of the zebrafish orthologues of mammalian MAP1LC3 gene family. Experimental cell research. 328(1):228-37
Newman, M., Ebrahimie, E., Lardelli, M. (2014) Using the zebrafish model for Alzheimer's disease research. Frontiers in genetics. 5:189
Moussavi Nik, S.H., Croft, K., Mori, T.A., Lardelli, M. (2014) The Comparison of Methods for Measuring Oxidative Stress in Zebrafish Brains. Zebrafish. 11(3):248-54
Newman, M., Wilson, L., Verdile, G., Lim, A., Khan, I., Moussavi Nik, S.H., Pursglove, S., Chapman, G., Martins, R.N., and Lardelli, M. (2014) Differential, dominant activation and inhibition of Notch signalling and APP cleavage by truncations of PSEN1 in human disease. Human molecular genetics. 23(3):602-17
Wilson, L., and Lardelli, M. (2013) The Development of an in vivo gamma-Secretase Assay using Zebrafish Embryos. Journal of Alzheimer's disease : JAD. 36(3):521-34
Avdesh, A., Chen, M., Martin-Iverson, M.T., Mondal, A., Ong, D., Rainey-Smith, S., Taddei, K., Lardelli, M., Groth, D.M., Verdile, G., and Martins, R.N. (2012) Regular Care and Maintenance of a Zebrafish (Danio rerio) Laboratory: An Introduction. Journal of visualized experiments : JoVE. (69):e4196
Newman, M., Nornes, S., Martins, R.N., and Lardelli, M.T. (2012) Robust homeostasis of Presenilin1 protein levels by transcript regulation. Neuroscience letters. 519(1):14-19
Nik, S.H., Wilson, L., Newman, M., Croft, K., Mori, T.A., Musgrave, I., and Lardelli, M. (2012) The BACE1-PSEN-AbetaPP Regulatory Axis has an Ancient Role in Response to Low Oxygen/Oxidative Stress. Journal of Alzheimer's disease : JAD. 28(3):515-530
Avdesh, A., Martin-Iverson, M.T., Mondal, A., Chen, M., Askraba, S., Newman, M., Lardelli, M., Groth, D.M., Verdile, G., and Martins, R.N. (2012) Evaluation of Color Preference in Zebrafish for Learning And Memory. Journal of Alzheimer's disease : JAD. 28(2):459-469
Sugano, Y., and Lardelli, M. (2011) Identification and expression analysis of the zebrafish orthologue of Klotho. Development genes and evolution. 221(3):179-86
Moussavi Nik, S.H., Newman, M., and Lardelli, M. (2011) The response of HMGA1 to changes in oxygen availability is evolutionarily conserved. Experimental cell research. 317(11):1503-1512
Newman, M., Verdile, G., Martins, R.N., and Lardelli, M. (2011) Zebrafish as a tool in Alzheimer's disease research. Biochimica et biophysica acta. Molecular basis of disease. 1812(3):346-352
Wells, S., Nornes, S., and Lardelli, M. (2011) Transgenic Zebrafish Recapitulating tbx16 Gene Early Developmental Expression. PLoS One. 6(6):e21559
Wells, S., Conran, J.G., Tamme, R., Gaudin, A., Webb, J., and Lardelli, M. (2010) Cryptic organisation within an apparently irregular rostrocaudal distribution of interneurons in the embryonic zebrafish spinal cord. Experimental cell research. 316(19):3292-3303
Newman, M., and Lardelli, M. (2010) A hyperactive sleeping beauty transposase enhances transgenesis in zebrafish embryos. BMC research notes. 3:282
Newman, M., Wilson, L., Camp, E., Verdile, G., Martins, R., and Lardelli, M. (2010) A zebrafish melanophore model of amyloid beta toxicity. Zebrafish. 7(2):155-159
Henshall, T.L., Tucker, B., Lumsden, A.L., Nornes, S., Lardelli, M.T., and Richards, R.I. (2009) Selective neuronal requirement for Huntingtin in the developing zebrafish. Human molecular genetics. 18(24):4830-4842
Nornes, S., Tucker, B., and Lardelli, M. (2009) Zebrafish aplnra functions in epiboly. BMC research notes. 2:231
Nornes, S., Newman, M., Wells, S., Verdile, G., Martins, R.N., and Lardelli, M. (2009) Independent and cooperative action of Psen2 with Psen1 in zebrafish embryos. Experimental cell research. 315(16):2791-2801
Newman, M., Tucker, B., Nornes, S., Ward, A., and Lardelli, M. (2009) Altering presenilin gene activity in zebrafish embryos causes changes in expression of genes with potential involvement in Alzheimer's disease pathogenesis. Journal of Alzheimer's disease : JAD. 16(1):133-147
Chen, M., Martins, R.N., and Lardelli, M. (2009) Complex Splicing and Neural Expression of Duplicated Tau Genes in Zebrafish Embryos. Journal of Alzheimer's disease : JAD. 18(2):305-317
Nornes, S., Newman, M., Verdile, G., Wells, S., Stoick-Cooper, C., Tucker, B., Frederich-Sleptsova, I., Martins, R., and Lardelli, M. (2008) Interference with splicing of Presenilin transcripts has potent dominant negative effects on Presenilin activity. Human molecular genetics. 17(3):402-412
Wyatt, L., Wadham, C., Crocker, L.A., Lardelli, M., and Khew-Goodall, Y. (2007) The protein tyrosine phosphatase Pez regulates TGF{beta}, epithelial mesenchymal transition, and organ development. The Journal of cell biology. 178(7):1223-1235
Lumsden, A.L., Henshall, T.L., Dayan, S., Lardelli, M.T., and Richards, R.I. (2007) Huntingtin-deficient zebrafish exhibit defects in iron utilization and development. Human molecular genetics. 16(16):1905-1920
Tucker, B., and Lardelli, M. (2007) A Rapid Apoptosis Assay Measuring Relative Acridine Orange Fluorescence in Zebrafish Embryos. Zebrafish. 4(2):113-116
Khut, P.Y., Tucker, B., Lardelli, M., and Wood, S.A. (2007) Evolutionary and Expression Analysis of the Zebrafish Deubiquitylating Enzyme, Usp9. Zebrafish. 4(2):95-101
Newman, M., Musgrave, F.I., and Lardelli, M. (2007) Alzheimer disease: Amyloidogenesis, the presenilins and animal models. Biochimica et biophysica acta. Molecular basis of disease. 1772(3):285-297
Tucker, B., Richards, R.I., and Lardelli, M. (2006) Contribution of mGluR and Fmr1 Functional Pathways to Neurite Morphogenesis, Craniofacial Development and Fragile X Syndrome. Human molecular genetics. 15(23):3446-3458
Tucker, B., Richards, R., and Lardelli, M. (2004) Expression of three zebrafish orthologs of human FMR1-related genes and their phylogenetic relationships. Development genes and evolution. 214(11):567-574
Scholpp, S., Groth, C., Lohs, C., Lardelli, M., and Brand, M. (2004) Zebrafish fgfr1 is a member of the fgf8 synexpression group and is required for fgf8 signalling at the midbrain-hindbrain boundary. Development genes and evolution. 214(6):285-295
Lardelli, M. (2003) The evolutionary relationships of zebrafish genes tbx6, tbx16/ spadetail and mga. Development genes and evolution. 213(10):519-522
Nornes, S., Groth, C., Camp, E., Ey, P., and Lardelli, M. (2003) Developmental control of Presenilin1 expression, endoproteolysis, and interaction in zebrafish embryos. Experimental cell research. 289(1):124-132
Camp, E., Badhwar, P., Mann, G.J., and Lardelli, M. (2003) Expression analysis of a tyrosinase promoter sequence in zebrafish. Pigment cell research. 16(2):117-126
Camp, E., Hope, R., Kortschak, R.D., Cox, T.C., and Lardelli, M. (2003) Expression of three spalt (sal) gene homologues in zebrafish embryos. Development genes and evolution. 213(1):35-43
Groth, C., Nornes, S., McCarty, R., Tamme, R., and Lardelli, M. (2002) Identification of a second presenilin gene in zebrafish with similarity to the human Alzheimer's disease gene presenilin2. Development genes and evolution. 212(10):486-490
Tamme, R., Wells, S., Conran, J.G., Lardelli, M. (2002) The identity and distribution of neural cells expressing the mesodermal determinant spadetail. BMC Developmental Biology. 2:9
Lardelli, M. (2002) Direct Submission of Mapping Data (Michael Lardelli). Direct Submission of Mapping Data.
Tamme, R., Mills, K., Rainbird, B., Nornes, S., and Lardelli, M. (2001) Simple, directional cDNA cloning for in situ transcript hybridization screens. Biotechniques. 31(4):938-942
Kortschak, R.D., Tamme, R., and Lardelli, M. (2001) Evolutionary analysis of vertebrate Notch genes. Development genes and evolution. 211(7):350-354
Camp, E. and Lardelli, M. (2001) Tyrosinase gene expression in zebrafish embryos. Development genes and evolution. 211(3):150-153
Tamme, R., Camp, E., Kortschak, R.D., and Lardelli, M. (2000) Nonspecific, nested suppression PCR method for isolation of unknown flanking DNA. Biotechniques. 28(5):895-899
Zhang, H.Y., Lardelli, M., and Ekblom, P. (1997) Sequence of zebrafish fibulin-1 and its expression in developing heart and other embryonic organs. Development genes and evolution. 207(5):340-351
Westin, J. and Lardelli, M. (1997) Three novel Notch genes in zebrafish: implications for vertebrate Notch gene evolution and function. Development genes and evolution. 207:51-63

NON-ZEBRAFISH PUBLICATIONS
Johnson, M. A.; Misra, R.M.; Lardelli, M.; Messina, M.; Ephraums, C.; Reeves, P.R.; Bolcevic, Z.; Noel, J.S.; Hum, C.P.; Mai H.V., Dyall-Smith, M.L. and Holmes, I.H. (1989) Synthesis in Escherichia coli of the major glycoprotein of human rotavirus: analysis of the antigenic regions. Gene 84: 73-81.

Rushlow, C.A., Hogan, A., Pinchin, S.M., Howe, K.M., Lardelli, M., Ish-Horowicz, D. (1989) The Drosophila hairy protein acts in both segmentation and bristle patterning and shows homology to N-myc. Embo J. 8: 3095-103.

Lardelli, M. (1991) The regulation and function of the pair-rule gene hairy in Drosophila melanogaster. Ph.D. thesis, Council for National Academic Awards, U.K..

Lardelli, M.T. and Ish-Horowicz, D. (1993) The Drosophilahairy pair-rule gene regulates embryonic patterning outside its apparent stripe domains. Development 118: 255-66.

Lardelli, M. and Lendahl, U. (1993) Motch A and MotchB - two mouse Notch homologues coexpressed in a wide variety of tissues. Exp. Cell Res. 204: 364-72.

Lardelli, M. and Lendahl, U. (1994) Generating bacteriophage lambda sublibraries enriched for rare clones. BioTechniques 16: 420-22.

Lardelli, M., Dahlstrand, J. and Lendahl, U. (1994) The novel Notch homologue mouse Notch 3 lacks specific EGF-repeats and is expressed in
proliferating neuroepithelium. Mech. Dev. 46: 123-36.

Larsson, C., Lardelli, M., White, I. and Lendahl, U. (1994) The human NOTCH 1, 2 and 3 genes are located at chromosome positions 9q34, 1p13-p11 and 19p13.2-13.1 in regions of neoplasia-associated translocation. Genomics 24: 253-8.

Barlow, C., Meister, B., Lardelli, M., Lendahl, U. and Vennström, B. (1994) Thyroid Abnormalities and Hepatocellular carcinoma in mice transgenic for v-erbA. EMBO J. 13: 4241-50.

Dahlstrand, J., Lardelli, M. and Lendahl, U. (1995) Nestin mRNA expression correlates with the CNS progenitor cell state in many, but not all, regions of developing CNS. Dev. Brain Res. 84: 109-129.

Mitsiadis, T. A., Lardelli, M., Lendahl, U. and Thesleff, I. (1995) Expression of Notch 1, 2 and 3 is regulated by epithelial-mesenchymal interactions and retinoic acid in the developing mouse tooth and associated with determination of ameloblast cell fate. J. Cell Biol. 130: 407-18.

Williams, R., Lendahl, U. and Lardelli, M. (1995) Complementary and combinatorial patterns of Notch gene family expression during early mouse development. Mech. Dev. 53: 357-68.

Lardelli, M., Williams, R. and Lendahl, U. (1995) Notch-related genes in animal development. Int. J. Dev. Biol. 39: 769-80.

Lardelli, M., Williams, R., Mitsiadis, T. A. and Lendahl, U. (1996) Expression of the Notch 3 intracellular domain in the early mouse CNS leads to an expansion of the CNS progenitor cell population and disturbed neural tube development. Mech. Dev. 59: 177-90.

Lardelli, M. (1997) Generation and PCR-screening of bacteriophage lambda sublibraries enriched for rare clones (the "sublibrary method"). in Methods in Molecular Biology, Vol. 67: PCR Cloning Protocols: From Molecular Cloning to Genetic Engineering, pages 345-55, Edited by B. A. White, Humana Press Inc., Totowa, N.J., U.S.A.

Tamme, R., Camp, E., Kortschak, R.D. and Lardelli, M. (2000) Non-specific, nested suppression PCR method for isolation of unknown flanking DNA. BioTechniques 28: 895-902.

Holland, L.Z., Abi Rached, Tamme, R., L., Holland, N.D., Kortschak, R.D., Inoko, H., Shiina, T., Burgtorf, C. and Lardelli, M. (2001) Characterization and developmental expression of the amphioxus homolog of Notch (AmphiNotch): Evolutionary conservation of multiple expression domains in amphioxus and vertebrates. Dev. Biol. 232:493-507.

Jönsson, J.-I., Xiang, Z., Pettersson, M., Lardelli, M. and Nilsson, G. (2001) Distinct and regulated expression of Notch receptors in hematopoietic lineages and during myeloid differentiation. Eur J Immunol. 31: 3240-7.

Groth, C and Lardelli, M. (2002) The structure and function of vertebrate fibroblast growth factor receptor 1. Int. J. Dev. Biol. 46: 393-400.

Lardelli, M. (2002) Nonspecific, nested suppression PCR method for isolation of unknown flanking DNA (“Cold-start method”) in Methods in Molecular Biology, Vol. 192: PCR Cloning Protocols, 2nd. Ed., pages 285-99, Edited by B.-Y. Chen and H. W. Janes, Humana Press Inc., Totowa, N.J., U.S.A.

Lardelli, M. (2002) Generation and PCR-screening of bacteriophage lambda sublibraries enriched for rare clones (the "sublibrary method"). in Methods in Molecular Biology, Vol. 192: PCR Cloning Protocols, 2nd. Ed., pages 391-9, Edited by B.-Y. Chen and H. W. Janes, Humana Press Inc., Totowa, N.J., U.S.A.