PUBLICATION

Gradual recruitment and selective clearing generate germ plasm aggregates in the zebrafish embryo

Authors
Eno, C., Pelegri, F.
ID
ZDB-PUB-140513-162
Date
2013
Source
Bioarchitecture   3: 125-32 (Journal)
Registered Authors
Pelegri, Francisco
Keywords
Birc5b, chromosomal passenger complex, cytokinesis, germ cell, germ plasm, maternal inheritance, microtubule asters, ribonucleoparticles, zebrafish
MeSH Terms
  • Cytoplasm/physiology*
  • Embryonic Development
  • Zebrafish/embryology*
  • Animals
  • Germ Cells/physiology*
PubMed
24721731 Full text @ Bioarchitecture
Abstract
Determination of primordial germ cells (PGCs) is one of the earliest decisions in animal embryogenesis. In many species, PGCs are determined through maternally-inherited germ plasm ribonucleoparticles (RNPs). In zebrafish, these are transmitted during oogenesis as dispersed RNPs, which after fertilization multimerize and become recruited as large aggregates at furrows for the first and second cell cycles. Here, we show that the number of recruited germ plasm RNPs is halved every cell cycle. We also show that germ plasm RNPs are recruited during the third cell cycle, but only transiently. Our data support a mechanism in which systematic local gathering of germ plasm RNPs during cytokinesis and threshold-dependent clearing contribute to forming germ plasm aggregates with the highest RNP number and germ cell-inducing potential.
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Citation
Eno, C., Pelegri, F. (2013) Gradual recruitment and selective clearing generate germ plasm aggregates in the zebrafish embryo. Bioarchitecture. 3:125-32.