An evolutionarily conserved long noncoding RNA TUNA controls pluripotency and neural lineage commitment
- Authors
- Lin, N., Chang, K.Y., Li, Z., Gates, K., Rana, Z.A., Dang, J., Zhang, D., Han, T., Yang, C.S., Cunningham, T.J., Head, S.R., Duester, G., Dong, P.D., and Rana, T.M.
- ID
- ZDB-PUB-140509-3
- Date
- 2014
- Source
- Molecular Cell 53(6): 1005-1019 (Journal)
- Registered Authors
- Dong, P. Duc, Gates, Keith, Zhang, Danhua
- Keywords
- none
- MeSH Terms
-
- Severity of Illness Index
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism
- Amino Acid Sequence
- RNA, Long Noncoding/genetics*
- RNA, Long Noncoding/metabolism
- Neurons/cytology
- Neurons/metabolism*
- RNA-Binding Proteins/genetics
- RNA-Binding Proteins/metabolism
- Fibroblast Growth Factor 4/genetics
- Fibroblast Growth Factor 4/metabolism
- Huntington Disease/genetics*
- Huntington Disease/metabolism
- Huntington Disease/pathology
- Motor Activity
- Sequence Homology, Amino Acid
- Animals
- Pluripotent Stem Cells/cytology
- Pluripotent Stem Cells/metabolism*
- SOXB1 Transcription Factors/genetics
- SOXB1 Transcription Factors/metabolism
- Conserved Sequence
- Cell Differentiation
- Molecular Sequence Data
- Gene Expression Regulation, Developmental*
- Humans
- Zebrafish/genetics*
- Zebrafish/growth & development
- Zebrafish/metabolism
- Promoter Regions, Genetic
- Signal Transduction
- Biological Evolution
- Mice
- Embryonic Stem Cells/cytology
- Embryonic Stem Cells/metabolism
- PubMed
- 24530304 Full text @ Mol. Cell
Here, we generated a genome-scale shRNA library targeting long intergenic noncoding RNAs (lincRNAs) in the mouse. We performed an unbiased loss-of-function study in mouse embryonic stem cells (mESCs) and identified 20 lincRNAs involved in the maintenance of pluripotency. Among these, TUNA (Tcl1 Upstream Neuron-Associated lincRNA, or megamind) was required for pluripotency and formed a complex with three RNA-binding proteins (RBPs). The TUNA-RBP complex was detected at the promoters of Nanog, Sox2, and Fgf4, and knockdown of TUNA or the individual RBPs inhibited neural differentiation of mESCs. TUNA showed striking evolutionary conservation of both sequence- and CNS-restricted expression in vertebrates. Accordingly, knockdown of tuna in zebrafish caused impaired locomotor function, and TUNA expression in the brains of Huntington’s disease patients was significantly associated with disease grade. Our results suggest that the lincRNA TUNA plays a vital role in pluripotency and neural differentiation of ESCs and is associated with neurological function of adult vertebrates.