PUBLICATION

Dynamic neuroanatomy at subcellular resolution in the zebrafish

Authors
Faucherre, A., and López-Schier, H.
ID
ZDB-PUB-131009-3
Date
2014
Source
Methods in molecular biology (Clifton, N.J.)   1082: 187-195 (Chapter)
Registered Authors
Faucherre, Adele, Lopez-Schier, Hernan
Keywords
neuroanatomy, zebrafish, live imaging, microscopy, fluorescent protein
MeSH Terms
  • Animals
  • Axons/metabolism
  • Cell Membrane/metabolism
  • Imaging, Three-Dimensional
  • Intracellular Space/metabolism*
  • Luminescent Proteins/genetics
  • Neuroanatomy/economics
  • Neuroanatomy/methods*
  • Neurons, Afferent/cytology
  • Time Factors
  • Zebrafish/anatomy & histology*
  • Zebrafish/embryology
PubMed
24048935 Full text @ Meth. Mol. Biol.
Abstract

Genetic means to visualize and manipulate neuronal circuits in the intact animal have revolutionized neurobiology. “Dynamic neuroanatomy” defines a range of approaches aimed at quantifying the architecture or subcellular organization of neurons over time during their development, regeneration, or degeneration. A general feature of these approaches is their reliance on the optical isolation of defined neurons in toto by genetically expressing markers in one or few cells. Here we use the afferent neurons of the lateral line as an example to describe a simple method for the dynamic neuroanatomical study of axon terminals in the zebrafish by laser-scanning confocal microscopy.

Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes