PUBLICATION

Soybean meal induces intestinal inflammation in zebrafish larvae

Authors
Hedrera, M.I., Galdames, J.A., Jimenez-Reyes, M.F., Reyes, A.E., Avendaño-Herrera, R., Romero, J., and Feijóo, C.G.
ID
ZDB-PUB-130816-12
Date
2013
Source
PLoS One   8(7): e69983 (Journal)
Registered Authors
Feijoo, Carmen G., Galdames, Jorge, Hedrera, Manuel
Keywords
none
MeSH Terms
  • Animal Nutritional Physiological Phenomena
  • Animals
  • Cell Movement
  • Diet
  • Disease Models, Animal
  • Enteritis/etiology
  • Enteritis/immunology
  • Enteritis/pathology
  • Enteritis/veterinary*
  • Fish Diseases/etiology
  • Fish Diseases/immunology*
  • Fish Diseases/pathology
  • Intestines/immunology
  • Intestines/pathology
  • Larva
  • Neutrophils/physiology
  • Saponins/administration & dosage
  • Saponins/adverse effects
  • Soybean Proteins/administration & dosage
  • Soybean Proteins/adverse effects
  • Soybean Proteins/immunology
  • Soybeans/adverse effects*
  • Soybeans/immunology
  • Zebrafish/immunology*
PubMed
23894568 Full text @ PLoS One
Abstract

The necessary replacement of fish meal with other protein source in diets of commercially important fish has prompted the study of the effect of the inclusion of different vegetable proteins sources on growth performance and on the gastro-intestinal tract. Currently, soybean meal is the primary protein source as a fish meal replacement because of its low price and high availability. Likewise, it is been documented that the ingestion of soybean meal by several fish species, such as salmonids and carp, triggers a type of intestinal inflammation called enteritis. In this paper, we analyzed the effects of the ingestion of soybean meal and two of its components, soy protein and soy saponin, on zebrafish to establish the basis for using zebrafish larvae as a model for fish nutrition. We took advantage of the existence of different transgenic lines, which allowed us to perform in vivo analysis. Our results indicated that larvae that were feed with soybean meal developed a clear intestinal inflammation as early as two day after beginning the diet. Moreover, we determined that is not the soy protein present in the diet but the soy saponin that is primarily responsible for triggering the immune response. These findings support the use of zebrafish screening assays to identify novel ingredients that would to improved current fish diets or would formulate new ones.

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