ZFIN ID: ZDB-PUB-130726-5
Cloning and promoter analysis of gonadotrophin-releasing hormone a in the orange-spotted grouper (Epinephelus coioides)
Chen, J., Luo, Y.S., Zhu, Z.Y., and Hu, W.
Date: 2013
Source: Yi chuan = Hereditas   35(7): 896-902 (Journal)
Registered Authors: Hu, Wei
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular*
  • Fish Proteins/chemistry
  • Fish Proteins/genetics*
  • Fish Proteins/metabolism
  • Gonadotropin-Releasing Hormone/chemistry
  • Gonadotropin-Releasing Hormone/genetics*
  • Gonadotropin-Releasing Hormone/metabolism
  • Humans
  • Molecular Sequence Data
  • Perciformes/classification
  • Perciformes/genetics*
  • Perciformes/metabolism
  • Promoter Regions, Genetic*
  • Sequence Alignment
PubMed: 23853361 Full text @ Yi Chuan
Gonadotrophin-releasing hormone (GnRH) is a key regulator of reproduction in all vertebrates. We first cloned the cDNA and genomic DNA sequences coding for GnRHa gene in the orange-spotted grouper (Epinephelis coioides), an economically important marine fish, and then cloned its promoter sequence. The region responsible for the cell-specific expression of GnRHa was located between -2005 bp to -956 bp from the translation start site. GnRHa promoter driven EGFP expression in transgenic zebrafish showed that GnRHa-positive neurons were primarily located in the midbrain and in the eyes. Our results provide an improved understanding of the regulatory mechanism and function of GnRHa of E. coioides.
Article is in Chinese.