|ZFIN ID: ZDB-PUB-130726-5|
Your Input Welcome
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.
Cloning and promoter analysis of gonadotrophin-releasing hormone a in the orange-spotted grouper (Epinephelus coioides)
Chen, J., Luo, Y.S., Zhu, Z.Y., and Hu, W.
|Source:||Yi chuan = Hereditas 35(7): 896-902 (Journal)|
|Registered Authors:||Hu, Wei|
|PubMed:||23853361 Full text @ Yi Chuan|
Chen, J., Luo, Y.S., Zhu, Z.Y., and Hu, W. (2013) Cloning and promoter analysis of gonadotrophin-releasing hormone a in the orange-spotted grouper (Epinephelus coioides). Yi chuan = Hereditas. 35(7):896-902.
ABSTRACTGonadotrophin-releasing hormone (GnRH) is a key regulator of reproduction in all vertebrates. We first cloned the cDNA and genomic DNA sequences coding for GnRHa gene in the orange-spotted grouper (Epinephelis coioides), an economically important marine fish, and then cloned its promoter sequence. The region responsible for the cell-specific expression of GnRHa was located between -2005 bp to -956 bp from the translation start site. GnRHa promoter driven EGFP expression in transgenic zebrafish showed that GnRHa-positive neurons were primarily located in the midbrain and in the eyes. Our results provide an improved understanding of the regulatory mechanism and function of GnRHa of E. coioides.
ADDITIONAL INFORMATION No data available
ERRATA and NOTESArticle is in Chinese.