PUBLICATION
In vivo mutation of pre-mRNA processing factor 8 (Prpf8) affects transcript splicing, cell survival and myeloid differentiation
- Authors
- Keightley, M.C., Crowhurst, M.O., Layton, J.E., Beilharz, T., Markmiller, S., Varma, S., Hogan, B.M., de Jong-Curtain, T.A., Heath, J.K., and Lieschke, G.J.
- ID
- ZDB-PUB-130708-8
- Date
- 2013
- Source
- FEBS letters 587(14): 2150-2157 (Journal)
- Registered Authors
- Crowhurst, Meredith, de Jong-Curtain, Tanya A., Heath, Joan K., Hogan, Ben M., Keightley, M. Cristina, Layton, Judy E., Lieschke, Graham J., Markmiller, Sebastian, Varma, Sony
- Keywords
- PRPF8, haematopoiesis, splicing, zebrafish, myelopoiesis, spliceosome, snRNP
- MeSH Terms
-
- Animals
- Codon, Nonsense*
- Zebrafish
- RNA Precursors/genetics
- RNA Precursors/metabolism
- Cell Differentiation*
- Genes, Lethal
- Myeloid Cells/physiology*
- RNA Splicing*
- Body Patterning/genetics
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Embryo, Nonmammalian/abnormalities
- Embryo, Nonmammalian/pathology
- Ribonucleoproteins, Small Nuclear/genetics
- Ribonucleoproteins, Small Nuclear/metabolism
- Cell Survival
- Humans
- Sequence Analysis, DNA
- Base Sequence
- RNA-Binding Proteins/genetics*
- RNA-Binding Proteins/metabolism
- PubMed
- 23714367 Full text @ FEBS Lett.
Citation
Keightley, M.C., Crowhurst, M.O., Layton, J.E., Beilharz, T., Markmiller, S., Varma, S., Hogan, B.M., de Jong-Curtain, T.A., Heath, J.K., and Lieschke, G.J. (2013) In vivo mutation of pre-mRNA processing factor 8 (Prpf8) affects transcript splicing, cell survival and myeloid differentiation. FEBS letters. 587(14):2150-2157.
Abstract
Mutated spliceosome components are recurrently being associated with perturbed tissue development and disease pathogenesis. CephalophOnus (cph), is a zebrafish mutant carrying an early premature STOP codon in the spliceosome component Prpf8 (pre-mRNA processing factor 8). Cph initially develops normally, but then develops widespread cell death, especially in neurons, and is embryonic lethal. Cph mutants accumulate aberrantly spliced transcripts retaining both U2- and U12-type introns. Within early haematopoiesis, myeloid differentiation is impaired, suggesting Prpf8 is required for haematopoietic development. Cph provides an animal model for zygotic PRPF8 dysfunction diseases and for evaluating therapeutic interventions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping