ZFIN ID: ZDB-PUB-130703-24
Expression of chondroitin/dermatan sulfate glycosyltransferases during early zebrafish development
Filipek-Górniok, B., Holmborn, K., Haitina, T., Habicher, J., Oliveira, M.B., Hellgren, C., Eriksson, I., Kjellén, L., Kreuger, J., and Ledin, J.
Date: 2013
Source: Developmental dynamics : an official publication of the American Association of Anatomists   242(8): 964-75 (Journal)
Registered Authors: Filipek-Górniok, Beata, Habicher, Judith, Holmborn, Katarina, Ledin, Johan
Keywords: chondroitin sulfate, polymerase, CSGALNACT, CHSY, CHPF, zebrafish
MeSH Terms:
  • Animals
  • Chondroitin
  • Chondroitin Sulfates/metabolism*
  • Dermatan Sulfate/metabolism*
  • Glycosyltransferases/classification
  • Glycosyltransferases/genetics
  • Glycosyltransferases/metabolism*
  • Phylogeny
  • Zebrafish
  • Zebrafish Proteins/classification
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 23703795 Full text @ Dev. Dyn.

Background: Chondroitin/dermatan sulfate (CS/DS) proteoglycans present in the extracellular matrix have important structural and regulatory functions. Results: Six human genes have previously been shown to catalyze CS/DS polymerization. Here we show that one of these genes, chpf, is represented by two copies in the zebrafish genome, chpfa and chpfb, while the other five human CS/DS glycosyltransferases csgalnact1, csgalnact2, chpf2, chsy1, and chsy3 all have single zebrafish orthologues. The putative zebrafish CS/DS glycosyltransferases are spatially and temporally expressed. Interestingly, overlapping expression of multiple glycosyltransferases coincides with high CS/DS deposition. Finally, whereas the relative levels of the related polysaccharide HS reach steady-state at around 2 days post fertilization, there is a continued relative increase of the CS amounts per larvae during the first 6 days of development, matching the increased cartilage formation. Conclusions: There are 7 CS/DS glycosyltransferases in zebrafish, which, based on homology, can be divided into the CSGALNACT, CHSY, and CHPF families. The overlap between intense CS/DS production and the expression of multiple CS/DS glycosyltransferases suggests that efficient CS/DS biosynthesis requires a combination of several glycosyltransferases.