PUBLICATION
Microinjecting holo-aequorin into dechorionated and intact zebrafish embryos
- Authors
- Webb, S.E., and Miller, A.L.
- ID
- ZDB-PUB-130605-9
- Date
- 2013
- Source
- Cold Spring Harbor protocols 2013(5): pdb.prot072967 (Journal)
- Registered Authors
- Miller, Andrew L., Webb, Sarah E.
- Keywords
- none
- MeSH Terms
-
- Aequorin/administration & dosage*
- Animals
- Calcium/analysis
- Developmental Biology/methods*
- Microinjections/methods*
- Signal Transduction
- Zebrafish/embryology*
- PubMed
- 23637360 Full text @ Cold Spring Harb. Protoc.
Citation
Webb, S.E., and Miller, A.L. (2013) Microinjecting holo-aequorin into dechorionated and intact zebrafish embryos. Cold Spring Harbor protocols. 2013(5):pdb.prot072967.
Abstract
The injection of holo-aequorin into embryos at the one-cell stage, along with the use of a simple photomultiplier tube or luminescence imaging system, allows transient localized elevations of free cytosolic Ca2+ to be recorded and observed during the first 24 h of zebrafish development. The technique for loading dechorionated or intact one-cell stage zebrafish embryos with holo-aequorin is described here.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping