ZFIN ID: ZDB-PUB-130211-4
Efficient genome editing in zebrafish using a CRISPR-Cas system
Hwang, W.Y., Fu, Y., Reyon, D., Maeder, M.L., Tsai, S.Q., Sander, J.D., Peterson, R.T., Yeh, J.R., and Joung, J.K.
Date: 2013
Source: Nat. Biotechnol.   31(3): 227-229 (Journal)
Registered Authors: Peterson, Randall, Yeh, Jing-Ruey (Joanna)
Keywords: none
MeSH Terms:
  • Animals
  • Base Sequence
  • DNA/genetics
  • DNA Cleavage
  • Embryo, Nonmammalian
  • Endonucleases/genetics
  • Genetic Engineering
  • Genome*
  • Inverted Repeat Sequences*
  • Molecular Sequence Data
  • Mutation
  • Nucleic Acid Conformation
  • RNA Editing
  • RNA, Guide/genetics*
  • Zebrafish/genetics*
PubMed: 23360964 Full text @ Nat. Biotechnol.

In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator–like effector nucleases.