ZFIN ID: ZDB-PUB-121019-11
Recovery of Pigmentation Following Selective Photothermolysis in Adult Zebrafish Skin: Clinical Implications for Laser Toning Treatment of Melasma
Kim, J.H., Kim, D.H., Kim, J.H., Lee, S.G., Kim, H.S., Park, H.C., and Kim, I.H.
Date: 2012
Source: Journal of cosmetic and laser therapy : official publication of the European Society for Laser Dermatology 14(6): 277-285 (Journal)
Registered Authors: Park, Hae-Chul
Keywords: laser, melanosome, photothermolysis, tyrosinase, zebrafish
MeSH Terms:
  • Animals
  • Arbutin/pharmacology
  • Humans
  • Lasers, Solid-State/therapeutic use*
  • Melanocytes/drug effects
  • Melanocytes/physiology*
  • Melanocytes/radiation effects
  • Melanosis/radiotherapy*
  • Melanosomes/drug effects
  • Melanosomes/physiology*
  • Melanosomes/radiation effects
  • Models, Animal
  • Monophenol Monooxygenase/antagonists & inhibitors
  • Monophenol Monooxygenase/metabolism
  • Phenylthiourea/pharmacology
  • Pyrones/pharmacology
  • Regeneration*/drug effects
  • Regeneration*/radiation effects
  • Skin/drug effects
  • Skin/metabolism
  • Skin/radiation effects
  • Skin Pigmentation*/drug effects
  • Skin Pigmentation*/radiation effects
  • Statistics, Nonparametric
  • Zebrafish
PubMed: 23057411 Full text @ J. Cosmet. Laser Ther.

In recent years, laser toning has gained popularity for the treatment of melasma, and tyrosinase inhibitors are conventionally used to prevent recurrence after this treatment. The effectiveness of this treatment modality, however, is still questionable, and additional in vivo studies are needed to validate the method. In this study, we used adult zebrafish skin as an adult melanocyte regenerative system and examined the simulated human skin response to laser toning. Melanosomes regenerated after selective photothermolysis, and these organelles showed a bi-directional translocation pattern in accordance with the changes of intact melanosome patterns. Furthermore, a tyrosinase inhibitor, 1-phenyl-2-thiourea (PTU), completely blocked melanosome regeneration after laser irradiation, but this inhibitor failed to prevent melanosome regeneration after the medication was discontinued. Finally, arbutin and kojic acid, the commercially available tyrosinase inhibitors, slowed down but did not completely block melanosome regeneration. Based on these findings, we describe the limitations of laser toning treatment of melasma and the combined use of tyrosinase inhibitors. We suggest that melanosomes in adult zebrafish skin can be utilized for studying melanosome regeneration response to laser irradiation and for developing a system to assess the comparative efficacy of melanogenic regulatory compounds.