PUBLICATION
Cyclic Caged Morpholinos: Conformationally Gated Probes of Embryonic Gene Function
- Authors
- Yamazoe, S., Shestopalov, I.A., Provost, E., Leach, S.D., and Chen, J.K.
- ID
- ZDB-PUB-120630-3
- Date
- 2012
- Source
- Angewandte Chemie (International ed. in English) 51(28): 6908-6911 (Journal)
- Registered Authors
- Chen, James K., Leach, Steven D., Shestopalov, Ilya
- Keywords
- antisense oligonucleotides, developmental biology, gene expression, oligonucleotides, zebrafish
- MeSH Terms
-
- Pancreas/embryology
- Pancreas/metabolism*
- Gene Expression Regulation, Developmental*
- Morpholinos/pharmacology*
- Cell Differentiation
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism
- Organogenesis
- Animals
- Larva/cytology
- Larva/metabolism*
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism*
- Oligonucleotides/pharmacology*
- Gene Expression*
- PubMed
- 22689470 Full text @ Angew. Chem. Int. Ed. Engl.
Citation
Yamazoe, S., Shestopalov, I.A., Provost, E., Leach, S.D., and Chen, J.K. (2012) Cyclic Caged Morpholinos: Conformationally Gated Probes of Embryonic Gene Function. Angewandte Chemie (International ed. in English). 51(28):6908-6911.
Abstract
Feeling a bit cagey: Morpholino-based antisense reagents have been caged through oligonucleotide cyclization (see scheme), enabling photocontrol of gene expression in zebrafish embryos and larvae. Using these reagents, the timing of exocrine cell fate commitment in the developing pancreas has been examined.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping