ZFIN ID: ZDB-PUB-120630-3
Cyclic Caged Morpholinos: Conformationally Gated Probes of Embryonic Gene Function
Yamazoe, S., Shestopalov, I.A., Provost, E., Leach, S.D., and Chen, J.K.
Date: 2012
Source: Angewandte Chemie (International ed. in English)   51(28): 6908-6911 (Journal)
Registered Authors: Chen, James K., Leach, Steven D., Shestopalov, Ilya
Keywords: antisense oligonucleotides, developmental biology, gene expression, oligonucleotides, zebrafish
MeSH Terms:
  • Animals
  • Cell Differentiation
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism*
  • Gene Expression*
  • Gene Expression Regulation, Developmental*
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Larva/cytology
  • Larva/metabolism*
  • Morpholinos/pharmacology*
  • Oligonucleotides/pharmacology*
  • Organogenesis
  • Pancreas/embryology
  • Pancreas/metabolism*
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism
PubMed: 22689470 Full text @ Angew. Chem. Int. Ed. Engl.

Feeling a bit cagey: Morpholino-based antisense reagents have been caged through oligonucleotide cyclization (see scheme), enabling photocontrol of gene expression in zebrafish embryos and larvae. Using these reagents, the timing of exocrine cell fate commitment in the developing pancreas has been examined.