PUBLICATION
Microarray-based gene profiling analysis of müller glia-derived retinal stem cells in light-damaged retinas from adult zebrafish
- Authors
- Qin, Z., and Raymond, P.A.
- ID
- ZDB-PUB-120630-2
- Date
- 2012
- Source
- Methods in molecular biology (Clifton, N.J.) 884: 255-261 (Chapter)
- Registered Authors
- Raymond, Pamela
- Keywords
- none
- MeSH Terms
-
- Zebrafish
- Flow Cytometry
- Stem Cells/metabolism*
- Animals
- Light/adverse effects*
- Oligonucleotide Array Sequence Analysis
- Neuroglia/metabolism*
- Retina/cytology
- Retina/injuries
- Retina/metabolism*
- Retina/radiation effects
- Gene Expression Profiling/methods*
- PubMed
- 22688712 Full text @ Meth. Mol. Biol.
Citation
Qin, Z., and Raymond, P.A. (2012) Microarray-based gene profiling analysis of müller glia-derived retinal stem cells in light-damaged retinas from adult zebrafish. Methods in molecular biology (Clifton, N.J.). 884:255-261.
Abstract
Microarray-based gene profiling has become an important technique to measure changes in gene expression on a genome-wide scale. Recently, cell-specific microarrays have been reported to study changes in gene expression of a particular cell type in several model organisms. Here, we describe a protocol to prepare RNA samples for microarray analysis of isolated Müller glia-derived retinal stem cells from light-damaged adult zebrafish expressing a fluorescent marker in Müller cells using enzymatic retinal dissociation followed by fluorescence-activated cell sorting (FACS).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping