PUBLICATION
High dynamic range optical projection tomography (HDR-OPT)
- Authors
- Fei, P., Yu, Z., Wang, X., Lu, P.J., Fu, Y., He, Z., Xiong, J., and Huang, Y.
- ID
- ZDB-PUB-120424-1
- Date
- 2012
- Source
- Optics express 20(8): 8824-8836 (Journal)
- Registered Authors
- Xiong, Jing-Wei
- Keywords
- none
- MeSH Terms
-
- Cartilage/embryology
- Algorithms
- Blood Vessels/embryology
- Tomography, Optical/instrumentation
- Tomography, Optical/methods*
- Tomography, Optical/statistics & numerical data
- Imaging, Three-Dimensional/statistics & numerical data
- Zebrafish/embryology
- Animals
- Optical Phenomena
- Image Processing, Computer-Assisted/statistics & numerical data
- Staining and Labeling
- PubMed
- 22513593 Full text @ Opt. Express
Citation
Fei, P., Yu, Z., Wang, X., Lu, P.J., Fu, Y., He, Z., Xiong, J., and Huang, Y. (2012) High dynamic range optical projection tomography (HDR-OPT). Optics express. 20(8):8824-8836.
Abstract
Traditional optical projection tomography (OPT) acquires a single image at each rotation angle, thereby suffering from limitations in CCD dynamic range; this conventional usage cannot resolve features in samples with highly heterogeneous absorption, such as in small animals with organs of varying size. We present a novel technique, applying multiple-exposure high dynamic range (HDR) imaging to OPT, and demonstrate its ability to resolve fine details in zebrafish embryos, without complicated chemical clearing. We implement the tomographic reconstruction algorithm on the GPU, yielding a performance increase of two orders of magnitude. These features give our method potential application in high-throughput, high-resolution in vivo 3D imaging.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping