Epidermal growth factor differentially regulates activin subunits in the zebrafish ovarian follicle cells via diverse signaling pathways
- Authors
- Chung, C.K., and Ge, W.
- ID
- ZDB-PUB-120417-5
- Date
- 2012
- Source
- Molecular and Cellular Endocrinology 361(1-2): 133-142 (Journal)
- Registered Authors
- Ge, Wei
- Keywords
- EGF, activin, ovary, zebrafish
- MeSH Terms
-
- p38 Mitogen-Activated Protein Kinases/metabolism
- Models, Biological
- Zebrafish/genetics
- Zebrafish/metabolism*
- Phosphatidylinositol 3-Kinases/metabolism
- Protein Kinases/metabolism
- Animals
- MAP Kinase Signaling System/drug effects
- Inhibin-beta Subunits/genetics
- Inhibin-beta Subunits/metabolism*
- Cells, Cultured
- Recombinant Proteins/pharmacology
- Humans
- Signal Transduction/drug effects*
- Female
- Epidermal Growth Factor/pharmacology*
- Ovarian Follicle/cytology*
- Ovarian Follicle/drug effects
- Ovarian Follicle/enzymology
- Ovarian Follicle/metabolism*
- PubMed
- 22503865 Full text @ Mol. Cell. Endocrinol.
Epidermal growth factor (EGF) promotes oocyte maturation in the zebrafish and its effect is mediated via the activin system. However, the mechanisms by which EGF regulates activin subunits in the follicle cells remain unknown. The present study demonstrated that EGF controlled expression of three activin subunits (inhbaa, inhbab and inhbb) in the follicle cells via diverse signaling pathways. The expression of inhbaa and inhbb was often co-regulated via similar pathways. Suppression of MAPK3/1, p38 MAPK, PKC and PKA each blocked or partially reduced the stimulatory effects of EGF on the expression of inhbaa and inhbb while up-regulated that of inhbab. Conversely, inhibition of PI3K did not have any effect on the expression of inhbaa and inhbb but significantly suppressed the stimulatory effect of EGF on inhbab. In summary, EGF action in the zebrafish ovary involves activin system and its regulation of activin subunits is mediated by diverse signaling pathways downstream of EGFR.