PUBLICATION

Zebrafish prox1b Mutants Develop a Lymphatic Vasculature, and prox1b Does Not Specifically Mark Lymphatic Endothelial Cells

Authors
Tao, S., Witte, M., Bryson-Richardson, R.J., Currie, P.D., Hogan, B.M., and Schulte-Merker, S.
ID
ZDB-PUB-120111-12
Date
2011
Source
PLoS One   6(12): e28934 (Journal)
Registered Authors
Bryson-Richardson, Robert, Currie, Peter D., Hogan, Ben M., Schulte-Merker, Stefan, Tao, Shijie, Witte, Merlijn
Keywords
none
MeSH Terms
  • Animals
  • Homeodomain Proteins/genetics*
  • Lymphangiogenesis
  • Lymphatic Vessels/cytology*
  • Mutation*
  • Tumor Suppressor Proteins/genetics*
  • Zebrafish/embryology
  • Zebrafish/genetics*
PubMed
22216143 Full text @ PLoS One
Abstract

Background

The expression of the Prospero homeodomain transcription factor (Prox1) in a subset of cardinal venous cells specifies the lymphatic lineage in mice. Prox1 is also indispensible for the maintenance of lymphatic cell fate, and is therefore considered a master control gene for lymphangiogenesis in mammals. In zebrafish, there are two prox1 paralogues, the previously described prox1 (also known as prox1a) and the newly identified prox1b.

Principal Findings

To investigate the role of the prox1b gene in zebrafish lymphangiogenesis, we knocked-down prox1b and found that depletion of prox1b mRNA did not cause lymphatic defects. We also generated two different prox1b mutant alleles, and maternal-zygotic homozygous mutant embryos were viable and did not show any lymphatic defects. Furthermore, the expression of prox1b was not restricted to lymphatic vessels during zebrafish development.

Conclusion

We conclude that Prox1b activity is not essential for embryonic lymphatic development in zebrafish.

Genes / Markers
Figures
Figure Gallery
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes