PUBLICATION
In vivo cell biology using Gal4-mediated multicolor subcellular labeling in zebrafish
- Authors
- Distel, M., Jennifer, C.H., and Köster, R.W.
- ID
- ZDB-PUB-111025-6
- Date
- 2011
- Source
- Communicative & integrative biology 4(3): 336-9 (Journal)
- Registered Authors
- Distel, Martin, Köster, Reinhard W.
- Keywords
- in vivo imaging, zebrafish, neuroepithelium, Gal4/UAS, sucellular labeling, mitosis
- MeSH Terms
- none
- PubMed
- 21980574 Full text @ Commun. Integr. Biol.
Citation
Distel, M., Jennifer, C.H., and Köster, R.W. (2011) In vivo cell biology using Gal4-mediated multicolor subcellular labeling in zebrafish. Communicative & integrative biology. 4(3):336-9.
Abstract
The behavior of a cell is determined by the interplay of its subcellular components. Thus, being able to simultaneously visualize several organelles inside cells within the natural context of a living organism could greatly enhance our understanding of developmental processes. We have established a Gal4-based system for the simultaneous and cell type specific expression of multiple subcellular labels in transparent zebrafish embryos. This system offers the opportunity to follow intracellular developmental processes in a live vertebrate organism using confocal fluorescence time-lapse microscopy. Using this approach we recently showed that the centrosome neither persistently leads migration nor determines the site of axonogenesis in migrating neurons in the zebrafish cerebellum in vivo. Here we present additional in vivo findings about the centrosomal and microtubule dynamics of neuroepithelial cells during mitotic cleavages at early neural tube stages.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping