|ZFIN ID: ZDB-PUB-111012-16|
Infectious disease modeling and innate immune function in zebrafish embryos
Cui, C., Benard, E.L., Kanwal, Z., Stockhammer, O.W., van der Vaart, M., Zakrzewska, A., Spaink, H.P., and Meijer, A.H.
|Source:||Methods in cell biology 105: 273-308 (Chapter)|
|Registered Authors:||Benard, Erica L., Kanwal, Zakia, Meijer, Annemarie H., Spaink, Herman P., Stockhammer, Oliver W., van der Vaart, Michiel, Zakrzewska, Ania|
|Keywords:||knockdown, innate, macrophage, mediator, neutrophil, reporter|
|PubMed:||21951535 Full text @ Meth. Cell. Biol.|
Cui, C., Benard, E.L., Kanwal, Z., Stockhammer, O.W., van der Vaart, M., Zakrzewska, A., Spaink, H.P., and Meijer, A.H. (2011) Infectious disease modeling and innate immune function in zebrafish embryos. Methods in cell biology. 105:273-308.
ABSTRACTThe major cell types of the innate immune system, macrophages and neutrophils, develop during the first two days of zebrafish embryogenesis. The interaction of these immune cells with pathogenic microbes can excellently be traced in the optically transparent zebrafish embryos. Various tools and methods have recently been developed for visualizing and isolating the zebrafish embryonic innate immune cells, for establishing infections by different micro-injection techniques, and for analyzing the host innate immune response following microbial recognition. Here we provide practical guidelines for the application of these methodologies and review the current state of the art in zebrafish infectious disease research.
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