PUBLICATION
Transgenic Zebrafish Recapitulating tbx16 Gene Early Developmental Expression
- Authors
- Wells, S., Nornes, S., and Lardelli, M.
- ID
- ZDB-PUB-110706-1
- Date
- 2011
- Source
- PLoS One 6(6): e21559 (Journal)
- Registered Authors
- Lardelli, Michael, Nornes, Svanhild
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism
- Fluorescence
- Gene Expression Regulation, Developmental*
- Genome/genetics
- Green Fluorescent Proteins/metabolism
- Interneurons/cytology
- Interneurons/metabolism
- Neurons/metabolism
- Organ Specificity/genetics
- Spinal Cord/cytology
- Spinal Cord/metabolism
- T-Box Domain Proteins/genetics*
- T-Box Domain Proteins/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 21720556 Full text @ PLoS One
Citation
Wells, S., Nornes, S., and Lardelli, M. (2011) Transgenic Zebrafish Recapitulating tbx16 Gene Early Developmental Expression. PLoS One. 6(6):e21559.
Abstract
We describe the creation of a transgenic zebrafish expressing GFP driven by a 7.5 kb promoter region of the tbx16 gene. This promoter segment is sufficient to recapitulate early embryonic expression of endogenous tbx16 in the presomitic mesoderm, the polster and, subsequently, in the hatching gland. Expression of GFP in the transgenic lines later in development diverges to some extent from endogenous tbx16 expression with the serendipitous result that one line expresses GFP specifically in commissural primary ascending (CoPA) interneurons of the developing spinal cord. Using this line we demonstrate that the gene mafba (valentino) is expressed in CoPA interneurons.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping