PUBLICATION
Analysis of cell proliferation, senescence, and cell death in zebrafish embryos
- Authors
- Verduzco, D., and Amatruda, J.F.
- ID
- ZDB-PUB-110520-27
- Date
- 2011
- Source
- Methods in cell biology 101: 19-38 (Chapter)
- Registered Authors
- Amatruda, James F., Verduzco, Daniel
- Keywords
- cell death, cell proliferation, differentiation, dysregulation, forward-genetic screen, senescence
- MeSH Terms
-
- Animals
- Cell Death/physiology
- Cell Growth Processes/physiology
- Cellular Senescence/physiology
- DNA Damage
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental
- Humans
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 21550438 Full text @ Meth. Cell. Biol.
Citation
Verduzco, D., and Amatruda, J.F. (2011) Analysis of cell proliferation, senescence, and cell death in zebrafish embryos. Methods in cell biology. 101:19-38.
Abstract
Proper control of cell proliferation is critical for normal development, growth, differentiation, and tissue homeostasis. Dysregulation of cell division and cell death underlies almost all cancers, and contributes to the pathology of birth defects and degenerative diseases. The zebrafish has proved to be an excellent system for elucidating the roles of the cell cycle in normal development, and ways in which dysregulation of cell proliferation contributes to disease. This chapter describes the methods for studying the cell cycle in zebrafish embryos, including protocols to examine cell proliferation, DNA damage, senescence, and cell death.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping