PUBLICATION
Conditioned place preference behavior in zebrafish
- Authors
- Mathur, P., Lau, B., and Guo, S.
- ID
- ZDB-PUB-110317-26
- Date
- 2011
- Source
- Nature Protocols 6(3): 338-345 (Journal)
- Registered Authors
- Guo, Su, Lau, Billy, Mathur, Priya
- Keywords
- Model organisms, Neuroscience
- MeSH Terms
-
- Animals
- Brain/metabolism
- Choice Behavior/drug effects*
- Conditioning, Classical/drug effects*
- Drug Evaluation, Preclinical/methods*
- Models, Animal
- Reward
- Time Factors
- Zebrafish
- PubMed
- 21372814 Full text @ Nat. Protoc.
Citation
Mathur, P., Lau, B., and Guo, S. (2011) Conditioned place preference behavior in zebrafish. Nature Protocols. 6(3):338-345.
Abstract
This protocol describes conditioned place preference (CPP) in zebrafish following a single exposure to a substance. In the CPP paradigm, animals show a preference for an environment that has previously been associated with a substance (drug), thus indicating the positive-reinforcing qualities of that substance. The test tank consists of two visually distinct compartments separated by a central alley. The protocol involves three steps: the determination of initial preference, one conditioning session and the determination of final preference. This procedure is carried out in ∼2 d; other reported CPP protocols take up to 2 weeks. An increase in preference for the drug-associated compartment is observed after a single exposure. Establishment of this high-throughput protocol in zebrafish makes it possible to investigate the molecular and cellular basis of choice behavior, reward and associative learning. The protocol is also a tool for testing psychoactive compounds in the context of a vertebrate brain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping