PUBLICATION
Labelling cell structures and tracking cell lineage in zebrafish using SNAP-tag
- Authors
- Campos, C., Kamiya, M., Banala, S., Johnsson, K., and González-Gaitán, M.
- ID
- ZDB-PUB-110317-11
- Date
- 2011
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 240(4): 820-827 (Journal)
- Registered Authors
- Keywords
- SNAP-tag, zebrafish, imaging, lineage tracing
- MeSH Terms
-
- Acids/pharmacology
- Animals
- Animals, Genetically Modified
- Cell Lineage*/genetics
- Cell Tracking/methods*
- Cellular Structures/cytology*
- Cellular Structures/metabolism
- Embryo, Nonmammalian/drug effects
- Fixatives/pharmacology
- Fluorescent Dyes/pharmacology*
- Fluorescent Dyes/toxicity
- Models, Biological
- Staining and Labeling/methods*
- Time-Lapse Imaging/methods
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish/physiology
- PubMed
- 21360787 Full text @ Dev. Dyn.
Citation
Campos, C., Kamiya, M., Banala, S., Johnsson, K., and González-Gaitán, M. (2011) Labelling cell structures and tracking cell lineage in zebrafish using SNAP-tag. Developmental Dynamics : an official publication of the American Association of Anatomists. 240(4):820-827.
Abstract
We present a method for the specific labelling of fusion proteins with synthetic fluorophores in Zebrafish. The method uses the SNAP-tag technology and O(6) -benzylguanine derivatives of various synthetic fluorophores. We demonstrate how the method can be used to label subcellular structures in Zebrafish such as the nucleus, cell membranes, and endosomal membranes. The stability of the synthetic fluorophores makes them attractive choices for long-term imaging and allows, unlike most of the autofluorescent proteins, the use of acid fixatives such as trichloroacetic acid. Furthermore, the use of O(6) -benzylguanine derivatives bearing caged fluorescein allows cell lineage tracing through photo-deprotection of the fluorophore and its detection either through fluorescence microscopy or through immunohistochemistry after fixation using anti-fluorescein antibodies. Developmental Dynamics 240:820-827, 2011. c. 2011 Wiley-Liss, Inc.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping