PUBLICATION
Discovery of zebrafish (Danio rerio) interleukin-23 alpha (IL-23α) chain, a subunit important for the formation of IL-23, a cytokine involved in the development of Th17 cells and inflammation
- Authors
- Holt, A., Mitra, S., van der Sar, A.M., Alnabulsi, A., Secombes, C.J., and Bird, S.
- ID
- ZDB-PUB-110221-15
- Date
- 2011
- Source
- Molecular immunology 48(8): 981-991 (Journal)
- Registered Authors
- van der Sar, Astrid M.
- Keywords
- Zebrafish, T-cells, IL-23α, p19, Comparative immunology, Molecular biology, Mycobacterium
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Chromosome Mapping
- Chromosomes, Human, Pair 12/genetics
- Cloning, Molecular
- Gene Expression Profiling
- Host-Pathogen Interactions
- Humans
- Inflammation Mediators/metabolism*
- Interleukin-23 Subunit p19/classification
- Interleukin-23 Subunit p19/genetics*
- Leukocytes/drug effects
- Leukocytes/metabolism
- Leukocytes/microbiology
- Lipopolysaccharides/pharmacology
- Molecular Sequence Data
- Mycobacterium marinum/physiology
- Phylogeny
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Synteny
- Th17 Cells/metabolism*
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- PubMed
- 21324528 Full text @ Mol. Immunol.
Citation
Holt, A., Mitra, S., van der Sar, A.M., Alnabulsi, A., Secombes, C.J., and Bird, S. (2011) Discovery of zebrafish (Danio rerio) interleukin-23 alpha (IL-23α) chain, a subunit important for the formation of IL-23, a cytokine involved in the development of Th17 cells and inflammation. Molecular immunology. 48(8):981-991.
Abstract
This paper reports the cloning and sequencing of interleukin (IL)-23 p19 subunit for the first time within a non-mammalian species, the zebrafish (Danio rerio), which was discovered using a synteny approach. In addition, amino acid sequences were for IL-23 p19 subunits were also predicted from the stickleback, Fugu and Tetraodon genomes and included in this investigation. The zebrafish IL-23 p19 cDNA consisted of a 66bp 5′ UTR, a 249bp 3′ UTR and a single open reading frame of 567bp giving a predicted 188 aa IL-23 p19 molecule. Multiple alignment of zebrafish IL-23 p19, with other known IL-23 p19 and IL-12 p35 amino acid sequences revealed areas of amino acid conservation, such as the presence of four predicted α-helixes, cysteines important for disulphide bond formation and the conservation of a tryptophan known to interact with the receptor. Amino acid homologies and phylogenetic analysis confirmed the relationship of the fish IL-23 p19 subunits with their mammalian homologues. All the teleost fish IL-23 p19 subunits were found to have 4 exons and 3 introns similar to that of human and mouse IL-23 p19 and a limited degree of synteny was found between the organisms for the regions containing the IL-23 p19 genes with only PAB-dependent poly(A)-specific ribonuclease subunit 2 (PAN2) and IL-23 p19 found in the same order on human chromosome 12 and all the fish genomes looked at. Lastly using real-time PCR, constitutive expression of IL-12 p40 and IL-23 p19 was observed in the kidney, liver, gut and muscle with IL-12 p40 expression higher than IL-23 p19. As soon as an hour after stimulation with LPS, there was an increase of IL-23 p19 in zebrafish leukocytes and an increase of IL-1β, IL-12 p40 and IL-23 p19 expression was found after infection of zebrafish for 1 or 6 days with Mycobaterium marinum strain E11.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping