PUBLICATION

Topographical distribution of antimicrobial genes in the zebrafish intestine

Authors
Oehlers, S.H., Flores, M.V., Chen, T., Hall, C.J., Crosier, K.E., and Crosier, P.S.
ID
ZDB-PUB-101201-4
Date
2011
Source
Developmental and comparative immunology   35(3): 385-391 (Journal)
Registered Authors
Crosier, Kathy, Crosier, Phil, Flores, Maria, Hall, Chris, Oehlers, Stefan
Keywords
Defensin, RNase-A, Peptidoglycan recognition protein, Innate immunity, Swim bladder, Zebrafish
MeSH Terms
  • Animals
  • Gene Expression
  • Gene Expression Profiling*
  • Immunity, Innate/immunology*
  • In Situ Hybridization
  • Intestines/immunology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Zebrafish/genetics*
  • Zebrafish/immunology*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/immunology
PubMed
21093479 Full text @ Dev. Comp. Immunol.
Abstract

The zebrafish is increasingly being utilized to study aspects of the conserved innate intestinal immunity of vertebrates. In mammals, some antimicrobial proteins are synthesised by specialised immune cells that appear to have no equivalent in zebrafish. To delineate foci of antimicrobial protein production along the zebrafish intestine, we examined the antero-posterior expression gradients of antimicrobial genes. Quantitative PCR revealed distinct expression gradient profiles, with the mid-intestine exhibiting elevated expression of several genes such as dual oxidase and the defensin beta-like and peptidoglycan recognition protein families. This region also presented with the most numbers of leukocytes and endocytic cells, supporting a specialised immunological role. Conversely, expression of the Dr-RNase family was prominent in the anterior intestine. Expression of the zebrafish β-defensin family was examined in adult zebrafish tissues. Strong expression of defensin beta-like 1 was detected in the swim bladder of zebrafish from the larval stage of development through to adults.

Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes