PUBLICATION
Preference for ethanol in zebrafish following a single exposure
- Authors
- Mathur, P., Berberoglu, M.A., and Guo, S.
- ID
- ZDB-PUB-101027-44
- Date
- 2011
- Source
- Behavioural brain research 217(1): 128-133 (Journal)
- Registered Authors
- Guo, Su, Mathur, Priya
- Keywords
- Ethanol, zebrafish, conditioned place preference (CPP), reinforcement pathway, addiction, reward
- MeSH Terms
-
- Animals
- Brain/metabolism
- Choice Behavior/drug effects*
- Conditioning, Psychological/drug effects
- Dose-Response Relationship, Drug
- Drug Evaluation, Preclinical/methods*
- Ethanol/pharmacokinetics
- Ethanol/pharmacology*
- Male
- Reward
- Zebrafish
- PubMed
- 20974186 Full text @ Behav. Brain Res.
Citation
Mathur, P., Berberoglu, M.A., and Guo, S. (2011) Preference for ethanol in zebrafish following a single exposure. Behavioural brain research. 217(1):128-133.
Abstract
Ethanol is one of the most widely abused drugs in the world. Its addictive property is believed to primarily stem from its ability to influence the brain reinforcement pathway evolved for mediating natural rewards. Although dopamine is a known component of the reinforcement pathway, clear molecular and cellular compositions of this pathway and its sensitivity to ethanol remain not well understood. Zebrafish has been increasingly used to model and understand human disease states, due to its genetic tractability and ease of maintenance. In this study, we determine whether adult zebrafish develop ethanol preference after a single exposure using a conditioned place preference (CPP) paradigm. Moreover, we establish a procedure that can be carried out in an automated and relatively high-throughput fashion. We find that zebrafish of the AB strain display significantly increased preference for the compartment where they received ethanol during a single 20 -minute exposure. The largest increase in preference is in response to a 1.5% ethanol administered in the tank water. The results demonstrate robust ethanol preference in zebrafish. Such a relatively high-throughput assay with automated tracking and response to a single ethanol exposure provides a potential means for a large-scale screening aimed at understanding the brain reinforcement pathway and its sensitivity to ethanol in this genetically tractable vertebrate.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping