PUBLICATION
Molecular cloning and embryonic expression of zebrafish PCSK5 co-orthologues: Functional assessment during lateral line development
- Authors
- Chitramuthu, B.P., Baranowski, D.C., Cadieux, B., Rousselet, E., Seidah, N.G., and Bennett, H.P.
- ID
- ZDB-PUB-101011-29
- Date
- 2010
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 239(11): 2933-2946 (Journal)
- Registered Authors
- Cadieux, Benoit
- Keywords
- zebrafish, microinjection, morpholino, PC5, lateral line neuromast, Iin situ hybridization, PCSK5.1, PCSK5.2
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Blotting, Northern
- Blotting, Western
- Embryo, Nonmammalian/metabolism
- Humans
- In Situ Hybridization
- Mice
- Molecular Sequence Data
- Proprotein Convertase 5/chemistry*
- Proprotein Convertase 5/genetics
- Proprotein Convertase 5/metabolism*
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Homology, Amino Acid
- Zebrafish
- Zebrafish Proteins/chemistry*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 20882679 Full text @ Dev. Dyn.
Citation
Chitramuthu, B.P., Baranowski, D.C., Cadieux, B., Rousselet, E., Seidah, N.G., and Bennett, H.P. (2010) Molecular cloning and embryonic expression of zebrafish PCSK5 co-orthologues: Functional assessment during lateral line development. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(11):2933-2946.
Abstract
Pro-protein convertase subtilisin/kexin 5 (PC5, also known as PC6) is a member of the subtilisin-like superfamily of serine proteases implicated in the maturation of latent precursor proteins into their functionally active derivatives. To investigate the functional roles, we have cloned the cDNA sequences encoding two candidate zebrafish PC5 convertases (designated as PCSK5.1 and PCSK5.2) co-orthologous to the single PC5 encoding gene (PCSK5) found in mammals. Both display syntenic correspondence to the human PCSK5 gene. Overall gene architecture has been conserved across species. While PC5.1 mRNA expression is very discrete within the otic vesicle and lateral line neuromasts, PC5.2 transcripts are more ubiquitously expressed within the central nervous system together with specific localization in various organs including liver, intestine, and otic vesicle. Zebrafish PC5.1-deficient embryos display abnormal neuromast deposition within the lateral line system and lack a normal touch response, consistent with the known sensory role that the lateral line plays in spatial awareness and sensing the environment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping