PUBLICATION
Intravenous microinjections of zebrafish larvae to study acute kidney injury
- Authors
- Cianciolo Cosentino, C., Roman, B.L., Drummond, I.A., and Hukriede, N.A.
- ID
- ZDB-PUB-100826-15
- Date
- 2010
- Source
- Journal of visualized experiments : JoVE (42): (Journal)
- Registered Authors
- Drummond, Iain, Hukriede, Neil, Roman, Beth
- Keywords
- none
- MeSH Terms
-
- Disease Models, Animal*
- Larva
- Male
- Animals
- Injections, Intravenous
- Zebrafish
- Acute Kidney Injury*
- Gentamicins/administration & dosage
- Microinjections/methods*
- Female
- PubMed
- 20729805 Full text @ J. Vis. Exp.
Citation
Cianciolo Cosentino, C., Roman, B.L., Drummond, I.A., and Hukriede, N.A. (2010) Intravenous microinjections of zebrafish larvae to study acute kidney injury. Journal of visualized experiments : JoVE. (42).
Abstract
In this video article we describe a zebrafish model of AKI using gentamicin as the nephrotoxicant. The technique consists of intravenous microinjections on 2 dpf zebrafish. This technique represents an efficient and rapid method to deliver soluble substances into the bloodstream of zebrafish larvae, allowing for the injection of 15-20 fish per hour. In addition to AKI studies, this microinjection technique can also be used for other types of experimental studies such as angiography. We provide a detailed protocol of the technique from equipment required to visual measures of decreased kidney function. In addition, we also demonstrate the process of fixation, whole mount immunohistochemistry with a kidney tubule marker, plastic embedding and sectioning of the larval zebrafish. We demonstrate that zebrafish larvae injected with gentamicin show morphological features consistent with AKI: edema, loss of cell polarity in proximal tubular epithelial cells, and morphological disruption of the tubule.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping