PUBLICATION

Intravenous microinjections of zebrafish larvae to study acute kidney injury

Authors
Cianciolo Cosentino, C., Roman, B.L., Drummond, I.A., and Hukriede, N.A.
ID
ZDB-PUB-100826-15
Date
2010
Source
Journal of visualized experiments : JoVE   (42): (Journal)
Registered Authors
Drummond, Iain, Hukriede, Neil, Roman, Beth
Keywords
none
MeSH Terms
  • Acute Kidney Injury*
  • Animals
  • Disease Models, Animal*
  • Female
  • Gentamicins/administration & dosage
  • Injections, Intravenous
  • Larva
  • Male
  • Microinjections/methods*
  • Zebrafish
PubMed
20729805 Full text @ J. Vis. Exp.
Abstract
In this video article we describe a zebrafish model of AKI using gentamicin as the nephrotoxicant. The technique consists of intravenous microinjections on 2 dpf zebrafish. This technique represents an efficient and rapid method to deliver soluble substances into the bloodstream of zebrafish larvae, allowing for the injection of 15-20 fish per hour. In addition to AKI studies, this microinjection technique can also be used for other types of experimental studies such as angiography. We provide a detailed protocol of the technique from equipment required to visual measures of decreased kidney function. In addition, we also demonstrate the process of fixation, whole mount immunohistochemistry with a kidney tubule marker, plastic embedding and sectioning of the larval zebrafish. We demonstrate that zebrafish larvae injected with gentamicin show morphological features consistent with AKI: edema, loss of cell polarity in proximal tubular epithelial cells, and morphological disruption of the tubule.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping