ZFIN ID: ZDB-PUB-100826-15
Intravenous microinjections of zebrafish larvae to study acute kidney injury
Cianciolo Cosentino, C., Roman, B.L., Drummond, I.A., and Hukriede, N.A.
Date: 2010
Source: Journal of visualized experiments : JoVE   (42): (Journal)
Registered Authors: Drummond, Iain, Hukriede, Neil, Roman, Beth
Keywords: none
MeSH Terms:
  • Acute Kidney Injury*
  • Animals
  • Disease Models, Animal*
  • Female
  • Gentamicins/administration & dosage
  • Injections, Intravenous
  • Larva
  • Male
  • Microinjections/methods*
  • Zebrafish
PubMed: 20729805 Full text @ J. Vis. Exp.
ABSTRACT
In this video article we describe a zebrafish model of AKI using gentamicin as the nephrotoxicant. The technique consists of intravenous microinjections on 2 dpf zebrafish. This technique represents an efficient and rapid method to deliver soluble substances into the bloodstream of zebrafish larvae, allowing for the injection of 15-20 fish per hour. In addition to AKI studies, this microinjection technique can also be used for other types of experimental studies such as angiography. We provide a detailed protocol of the technique from equipment required to visual measures of decreased kidney function. In addition, we also demonstrate the process of fixation, whole mount immunohistochemistry with a kidney tubule marker, plastic embedding and sectioning of the larval zebrafish. We demonstrate that zebrafish larvae injected with gentamicin show morphological features consistent with AKI: edema, loss of cell polarity in proximal tubular epithelial cells, and morphological disruption of the tubule.
ADDITIONAL INFORMATION