|ZFIN ID: ZDB-PUB-100826-15|
Intravenous microinjections of zebrafish larvae to study acute kidney injury
Cianciolo Cosentino, C., Roman, B.L., Drummond, I.A., and Hukriede, N.A.
|Source:||Journal of visualized experiments : JoVE (42): (Journal)|
|Registered Authors:||Drummond, Iain, Hukriede, Neil, Roman, Beth|
|PubMed:||20729805 Full text @ J. Vis. Exp.|
Cianciolo Cosentino, C., Roman, B.L., Drummond, I.A., and Hukriede, N.A. (2010) Intravenous microinjections of zebrafish larvae to study acute kidney injury. Journal of visualized experiments : JoVE. (42).
ABSTRACTIn this video article we describe a zebrafish model of AKI using gentamicin as the nephrotoxicant. The technique consists of intravenous microinjections on 2 dpf zebrafish. This technique represents an efficient and rapid method to deliver soluble substances into the bloodstream of zebrafish larvae, allowing for the injection of 15-20 fish per hour. In addition to AKI studies, this microinjection technique can also be used for other types of experimental studies such as angiography. We provide a detailed protocol of the technique from equipment required to visual measures of decreased kidney function. In addition, we also demonstrate the process of fixation, whole mount immunohistochemistry with a kidney tubule marker, plastic embedding and sectioning of the larval zebrafish. We demonstrate that zebrafish larvae injected with gentamicin show morphological features consistent with AKI: edema, loss of cell polarity in proximal tubular epithelial cells, and morphological disruption of the tubule.
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