PUBLICATION
Evaluation of pyridoacridine alkaloids in a zebrafish phenotypic assay
- Authors
- Wei, X., Bugni, T.S., Harper, M.K., Sandoval, I.T., Manos, E.J., Swift, J., Van Wagoner, R.M., Jones, D.A., and Ireland, C.M.
- ID
- ZDB-PUB-100719-64
- Date
- 2010
- Source
- Marine drugs 8(6): 1769-1778 (Journal)
- Registered Authors
- Keywords
- pyridoacridine alkaloids, Xestospongia cf. carbonaria, zebrafish
- MeSH Terms
-
- Acridines/chemistry*
- Acridines/isolation & purification
- Acridines/toxicity*
- Animals
- Cytotoxins/chemistry
- Cytotoxins/isolation & purification
- Cytotoxins/toxicity
- Drug Discovery/methods*
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/pathology
- Embryonic Development/drug effects
- In Situ Hybridization
- Notochord/drug effects
- Notochord/pathology
- Pacific Ocean
- Palau
- Phenanthrolines/chemistry*
- Phenanthrolines/isolation & purification
- Phenanthrolines/toxicity*
- Somites/drug effects
- Somites/pathology
- Teratogens/chemistry*
- Teratogens/isolation & purification
- Teratogens/toxicity*
- Tissue Extracts/chemistry
- Toxicity Tests
- Xestospongia/chemistry
- Zebrafish
- PubMed
- 20631869 Full text @ Mar. Drugs
Citation
Wei, X., Bugni, T.S., Harper, M.K., Sandoval, I.T., Manos, E.J., Swift, J., Van Wagoner, R.M., Jones, D.A., and Ireland, C.M. (2010) Evaluation of pyridoacridine alkaloids in a zebrafish phenotypic assay. Marine drugs. 8(6):1769-1778.
Abstract
Three new minor components, the pyridoacridine alkaloids 1-hydroxy-deoxyamphimedine (1), 3-hydroxy-deoxyamphimedine (2), debromopetrosamine (3), and three known compounds, amphimedine (4), neoamphimedine (5) and deoxyamphimedine (6), have been isolated from the sponge Xestospongia cf. carbonaria, collected in Palau. Structures were assigned on the basis of extensive 1D and 2D NMR studies as well as analysis by HRESIMS. Compounds 1-6 were evaluated in a zebrafish phenotype-based assay. Amphimedine (4) was the only compound that caused a phenotype in zebrafish embryos at 30 muM. No phenotype other than death was observed for compounds 1-3, 5, 6.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping