PUBLICATION
hand2 and Dlx genes specify dorsal, intermediate and ventral domains within zebrafish pharyngeal arches
- Authors
- Talbot, J.C., Johnson, S.L., and Kimmel, C.B.
- ID
- ZDB-PUB-100625-26
- Date
- 2010
- Source
- Development (Cambridge, England) 137(15): 2507-2517 (Journal)
- Registered Authors
- Johnson, Stephen L., Kimmel, Charles B., Talbot, Jared
- Keywords
- Dlx, Hand2, Joint, Patterning, Skeleton, Zebrafish
- MeSH Terms
-
- Animals
- Basic Helix-Loop-Helix Transcription Factors/metabolism*
- Body Patterning
- Bone and Bones/metabolism
- Branchial Region/embryology
- Branchial Region/physiology
- Gene Expression Regulation*
- Gene Expression Regulation, Developmental*
- Homeodomain Proteins/metabolism*
- Mutation
- Protein Structure, Tertiary
- Transcription Factors/metabolism*
- Zebrafish
- Zebrafish Proteins/metabolism*
- PubMed
- 20573696 Full text @ Development
Citation
Talbot, J.C., Johnson, S.L., and Kimmel, C.B. (2010) hand2 and Dlx genes specify dorsal, intermediate and ventral domains within zebrafish pharyngeal arches. Development (Cambridge, England). 137(15):2507-2517.
Abstract
The ventrally expressed secreted polypeptide endothelin1 (Edn1) patterns the skeleton derived from the first two pharyngeal arches into dorsal, intermediate and ventral domains. Edn1 activates expression of many genes, including hand2 and Dlx genes. We wanted to know how hand2/Dlx genes might generate distinct domain identities. Here, we show that differential expression of hand2 and Dlx genes delineates domain boundaries before and during cartilage morphogenesis. Knockdown of the broadly expressed genes dlx1a and dlx2a results in both dorsal and intermediate defects, whereas knockdown of three intermediate-domain restricted genes dlx3b, dlx4b and dlx5a results in intermediate-domain-specific defects. The ventrally expressed gene hand2 patterns ventral identity, in part by repressing dlx3b/4b/5a. The jaw joint is an intermediate-domain structure that expresses nkx3.2 and a more general joint marker, trps1. The jaw joint expression of trps1 and nkx3.2 requires dlx3b/4b/5a function, and expands in hand2 mutants. Both hand2 and dlx3b/4b/5a repress dorsal patterning markers. Collectively, our work indicates that the expression and function of hand2 and Dlx genes specify major patterning domains along the dorsoventral axis of zebrafish pharyngeal arches.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping