PUBLICATION
Characterizing Mechanical Properties of Biological Cells by Microinjection
- Authors
- Tan, Y., Sun, D., Huang, W., and Cheng, S.H.
- ID
- ZDB-PUB-100614-15
- Date
- 2010
- Source
- IEEE Transactions on Nanobioscience 9(3): 171-180 (Journal)
- Registered Authors
- Cheng, Shuk Han
- Keywords
- Biological system modeling, Biomembranes, Embryo, Mathematical model, Mechanical factors, Mechanical model, Microinjection, biological cells, constitutive material, mechanical properties, microinjection
- MeSH Terms
-
- Animals
- Biomechanical Phenomena/physiology*
- Cell Membrane/physiology*
- Cell Shape/physiology*
- Elastic Modulus
- Embryo, Mammalian
- Embryo, Nonmammalian
- Finite Element Analysis
- Mice
- Microinjections*
- Models, Biological*
- Reproducibility of Results
- Shear Strength
- Zebrafish
- PubMed
- 20525536 Full text @ IEEE Trans. Nanobioscience
Citation
Tan, Y., Sun, D., Huang, W., and Cheng, S.H. (2010) Characterizing Mechanical Properties of Biological Cells by Microinjection. IEEE Transactions on Nanobioscience. 9(3):171-180.
Abstract
Microinjection has been demonstrated to be an effective technique to introduce foreign materials into biological cells. Despite the advance, whether cell injection can be used to characterize the mechanical properties of cells remains elusive. In this paper, extending the previously developed mechanical model [1], various constitutive materials are adopted to present the membrane characteristics of cells. To demonstrate the modeling approach and identify the most appropriate constitutive material for a specific biomembrane, finite element analysis (FEA) and experimental tests are carried out. It is shown that the modeling results agree well with those from both FEA and experiments, which demonstrates the validity of the developed approach. Moreover, Yeoh and Cheng materials are found to be the best constitutive materials in representing the deformation behaviors of zebrafish embryos and mouse embryos (or oocytes), respectively. Also, the mechanical properties of zebrafish embryos at different developmental stages and mouse embryos (or oocytes) are characterized.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping