PUBLICATION

Gli2a protein localization reveals a role for Iguana/DZIP1 in primary ciliogenesis and a dependence of Hedgehog signal transduction on primary cilia in the zebrafish

Authors

Kim, H.R., Richardson, J., van Eeden, F., and Ingham, P.W.

ID

ZDB-PUB-100525-11

Date

2010

Source

BMC Biology 8: 65 (Journal)

Registered Authors

Ingham, Philip, Kim, Rosemary, van Eeden, Freek

Keywords

none

MeSH Terms

Signal Transduction/physiology*
Green Fluorescent Proteins/metabolism
Embryo, Nonmammalian/metabolism
Embryo, Nonmammalian/physiology
Transcription Factors/metabolism*
Zebrafish
Sequence Analysis, DNA
Immunohistochemistry
Recombinant Fusion Proteins/metabolism
DNA Primers/genetics
Amino Acid Sequence
In Situ Hybridization
Carrier Proteins/metabolism*
Animals
Zebrafish Proteins/metabolism*
Cilia/physiology*
Molecular Sequence Data
Hedgehog Proteins/metabolism*

PubMed

20487519 Full text @ BMC Biol.

Abstract

BACKGROUND: In mammalian cells, the integrity of the primary cilium is critical for proper regulation of the Hedgehog (Hh) signal transduction pathway. Whether or not this dependence on the primary cilium is a universal feature of vertebrate Hedgehog signalling has remained contentious due, in part, to the apparent divergence of the intracellular transduction pathway between mammals and teleost fish. RESULTS: Here, using a functional Gli2-GFP fusion protein, we show that, as in mammals, the Gli2 transcription factor localizes to the primary cilia of cells in the zebrafish embryo and that this localization is modulated by the activity of the Hh pathway. Moreover, we show that the Igu/DZIP1protein, previously implicated in the modulation of Gli activity in zebrafish, also localizes to the primary cilium and is required for its proper formation. CONCLUSION: Our findings demonstrate a conserved role of the primary cilium in mediating Hedgehog signalling activity across the vertebrate phylum and validate the use of the zebrafish as a representative model for the in vivo analysis of vertebrate Hedgehog signalling.

Genes / Markers

Figures