PUBLICATION

Photoactivation of the CreER(T2) Recombinase for Conditional Site-Specific Recombination with High Spatiotemporal Resolution

Authors
Sinha, D.K., Neveu, P., Gagey, N., Aujard, I., Le Saux, T., Rampon, C., Gauron, C., Kawakami, K., Leucht, C., Bally-Cuif, L., Volovitch, M., Bensimon, D., Jullien, L., and Vriz, S.
ID
ZDB-PUB-100511-8
Date
2010
Source
Zebrafish   7(2): 199-204 (Journal)
Registered Authors
Bally-Cuif, Laure, Kawakami, Koichi, Leucht, Christoph, Vriz, Sophie
Keywords
none
MeSH Terms
  • Microscopy, Fluorescence
  • Polymerase Chain Reaction
  • Gene Expression Regulation, Enzymologic/physiology*
  • Integrases/metabolism*
  • Animals, Genetically Modified
  • DNA Primers/genetics
  • Spectrometry, Fluorescence
  • Animals
  • Zebrafish*
  • HSP70 Heat-Shock Proteins/metabolism
  • Recombinant Fusion Proteins/metabolism*
  • Photochemical Processes
  • Recombination, Genetic/physiology*
(all 13)
PubMed
20441524 Full text @ Zebrafish
Abstract
We implemented a noninvasive optical method for the fast control of Cre recombinase in single cells of a live zebrafish embryo. Optical uncaging of the caged precursor of a nonendogeneous steroid by one- or two-photon illumination was used to restore Cre activity of the CreER(T2) fusion protein in specific target cells. This method labels single cells irreversibly by inducing recombination in an appropriate reporter transgenic animal and thereby can achieve high spatiotemporal resolution in the control of gene expression. This technique could be used more generally to investigate important physiological processes (e.g., in embryogenesis, organ regeneration, or carcinogenesis) with high spatiotemporal resolution (single cell and 10-min scales).
Genes / Markers
Figures
Figure Gallery (2 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ne2466TgTransgenic Insertion
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    No data available
    Fish
    Fish
    ne2466Tg
    1 - 1 of 1
    Show
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    DsRed2EFGDsRed2
    GFPEFGGFP
    1 - 2 of 2
    Show
    Mapping
    No data available
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    Citation
    Sinha, D.K., Neveu, P., Gagey, N., Aujard, I., Le Saux, T., Rampon, C., Gauron, C., Kawakami, K., Leucht, C., Bally-Cuif, L., Volovitch, M., Bensimon, D., Jullien, L., and Vriz, S. (2010) Photoactivation of the CreER(T2) Recombinase for Conditional Site-Specific Recombination with High Spatiotemporal Resolution. Zebrafish. 7(2):199-204.