PUBLICATION

Characterization of an lhx1a transgenic reporter in zebrafish

Authors
Swanhart, L.M., Takahashi, N., Jackson, R.L., Gibson, G.A., Watkins, S.C., Dawid, I.B., and Hukriede, N.A.
ID
ZDB-PUB-100309-27
Date
2010
Source
The International journal of developmental biology   54(4): 731-736 (Journal)
Registered Authors
Dawid, Igor B., Hukriede, Neil, Jackson, Rachel, Takahashi, Nobuhiro
Keywords
lhx1a, transgene, zebrafish, kidney, gastrulation
MeSH Terms
  • Transgenes
  • Regulatory Sequences, Nucleic Acid
  • Homeodomain Proteins/genetics*
  • Homeodomain Proteins/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Genes, Reporter*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Gastrula/metabolism
  • Animals
  • Green Fluorescent Proteins/genetics
  • LIM-Homeodomain Proteins
  • Animals, Genetically Modified
  • Dioxoles/metabolism
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism
  • Benzamides/metabolism
(all 18)
PubMed
20209443 Full text @ Int. J. Dev. Biol.
Abstract
The LIM-domain containing transcription factor, Lhx1, is involved in the regulation of early gastrulation cell movements, kidney organogenesis and other processes in vertebrate model organisms. To follow the expression of this gene in live embryos, we created transgenic zebrafish expressing enhanced green fluorescent protein (EGFP) under the control of lhx1a regulatory regions. Tg(lhx1a:EGFP) (pt303) recapitulates the expression of endogenous lhx1a beginning at early gastrula stages through 72 hours of development with only few exceptions. In addition, over-expression of the Nodal ligand, ndr1, results in the concomitant expansion of the transgene and endogenous lhx1a expression. Treatment of Tg(lhx1a:EGFP) (pt303) embryos with the small molecule SB-431542, an inhibitor of Nodal signaling, results in the loss of both transgene and endogenous lhx1a expression. These experiments suggest that Tg(lhx1a:EGFP) (pt303) is regulated in a manner similar to endogenous lhx1a. Therefore, this reporter can be utilized not only for monitoring lhx1a expression, but also for numerous applications, including chemical genetics screening.
Genes / Markers
Figures
Figure Gallery (4 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
pt301TgTransgenic Insertion
    pt302TgTransgenic Insertion
      pt303TgTransgenic Insertion
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        Fish
        pt301Tg/pt301Tg
        pt302Tg/pt302Tg
        pt303Tg/pt303Tg
        1 - 3 of 3
        Show
        Antibodies
        No data available
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        EGFPEFGEGFP
        1 - 1 of 1
        Show
        Mapping
        No data available
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        Citation
        Swanhart, L.M., Takahashi, N., Jackson, R.L., Gibson, G.A., Watkins, S.C., Dawid, I.B., and Hukriede, N.A. (2010) Characterization of an lhx1a transgenic reporter in zebrafish. The International journal of developmental biology. 54(4):731-736.